The Drosophila ACP65A cuticle gene:: Deletion scanning analysis of cis-regulatory sequences and regulation by DHR38

被引：17

作者：

Bruey-Sedano, N ^{[1
]}

Alabouvette, J ^{[1
]}

Lestradet, M ^{[1
]}

Hong, L ^{[1
]}

Girard, A ^{[1
]}

Gervasio, E ^{[1
]}

Quennedey, B ^{[1
]}

Charles, JP ^{[1
]}

机构：

[1] Univ Bourgogne, Fac Sci Gabriel, CNRS, UMR Dev Commun Chim 5548, F-21000 Dijon, France

来源：

GENESIS | 2005年 / 43卷 / 01期

关键词：

Drosophila; cuticle; ecdysteroids; juvenile hormones; NGFI-B; DHR38; ACP65A; epidermis;

D O I：

10.1002/gene.20150

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The regulatory sequences of the Drosophila ACP65A cuticle gene were analyzed in vivo in transgenic flies, using both fusion genes constructs and transposase-mediated deletions within a P element containing ACP65A regulatory sequences fused to the lacZ gene (deletion scanning). The sequences located between -594 and +161 are sufficient to confer both temporal and spatial expression specificities, indicating the presence of tissue-specific enhancers and response elements to hormone-induced factors. In addition, timing of expression and tissue-specificity appear to be controlled by distinct cis-regulatory elements, which suggests the existence of independent hormonal and tissue-specific signaling pathways. Gain and loss of function studies also implicate DHR38, the Drosophila homolog of the vertebrate NGFI-B-type nuclear receptors, as an important activator of the ACP65A gene.

引用

页码：17 / 27

页数：11

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