The serum albumin-binding region of streptococcal protein G (BB) potentiates the immunogenicity of the G130-230 RSV-A protein

被引：52

作者：

Libon, C

Corvaïa, N

Haeuw, JF

Nguyen, TN

Ståhl, S

Bonnefoy, JY

Andreoni, C

机构：

[1] Inst Rech Pierre Fabre, Ctr Immunol, F-74164 Saint Julien en Genevois, France

[2] Royal Inst Technol, Dept Biochem & Biotechnol, KTH, S-10044 Stockholm, Sweden

来源：

VACCINE | 1999年 / 17卷 / 05期

关键词：

RSV vaccine; antibody production; carrier protein;

D O I：

10.1016/S0264-410X(98)00198-4

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

BBG2Na is a protein comprising residues 130-230 of the respiratory syncytial virus subgroup A (RSV-A) G protein (G2Na) fused to the albumin-binding domain of streptococcal G protein (BB). BBG2Na was cloned, expressed in Escherichia coli and renaturated. In rodent models, this subunit RSV vaccine adjuvanted in Alhydrogel(R) induced specific antibodies and conferred protection to RSV infection. Comparison of the antibody production in a BALB/c mouse model revealed that BBG2Na induced a stronger and earlier G2Na antibody response than G2Na alone, without altering the Ige subclass distribution. To address the role of the BE part, we explored its carrier properties and showed that it is a Th dependent antigen, generating a more potent G2Na-specific B cell memory response and able to generate Th cells that provide help for G2Na antibody production. (C) 1999 Elsevier Science Ltd. ALI rights reserved.

引用

页码：406 / 414

页数：9

共 33 条

[1]

AKERSTROM B, 1987, J BIOL CHEM, V262, P13388

[2] MYCOBACTERIAL HEAT-SHOCK PROTEINS AS CARRIER MOLECULES .2. THE USE OF THE 70-KDA MYCOBACTERIAL HEAT-SHOCK PROTEIN AS CARRIER FOR CONJUGATED VACCINES CAN CIRCUMVENT THE NEED FOR ADJUVANTS AND BACILLUS-CALMETTE-GUERIN PRIMING [J].