Changes in the composition of an acid mine drainage microbial community upon successive transfers in medium containing low-grade copper sulfide

被引:15
作者
Liu, Yi
Yin, Huaqun
Liang, Yili
Shen, Li
Liu, Yao
Fu, Xian
Baba, Ngom
Zeng, Weimin
Qiu, Guanzhou
Liu, Xueduan [1 ]
机构
[1] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China
基金
中国国家自然科学基金;
关键词
Adaptation; Community structure and function; Functional gene arrays; RFLP; Bioleaching; BACTERIAL DIVERSITY; MICROORGANISMS; CHALCOPYRITE; FERROOXIDANS; GENES; RNA;
D O I
10.1016/j.biortech.2011.05.095
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
A consortium of microorganisms from acid mine drainage samples was cultured in modified 9 K medium containing low-grade copper sulfide. The culture was maintained for sixty days and then transferred to fresh medium. This process was repeated three more times and a final consortium exhibiting a copper extraction rate of 89.3% was obtained. RFLP and microarrays analysis of 16S rRNA sequences retrieved from the consortia showed that Acidithiobacillus caldus, Leptospirillum ferriphilum, Sulfobacillus sp., Acidiphilium sp., and Sulfolobus spp. were represented in higher numbers in the consortia obtained in the copper-containing medium than in the original consortium. In contrast, a decrease in Acidithiobacillus ferrooxidans, Alicyclobacillus sp., Pseudomonas sp., and Sulfobacillus thermosulfidooxidans was observed. The abundance of genes related to sulfur metabolism from At. caldus and Sulfolobus spp., iron oxidation from Leptospirillum sp. and metal resistance from most of the detected microorganisms increased as the consortium was successively transferred into fresh medium. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:9388 / 9394
页数:7
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