Human papillomavirus 16 E6 is associated with the nuclear matrix of esophageal carcinoma cells

被引:16
作者
Chen, HB
Chen, L
Zhang, JK
Shen, ZY
Su, ZJ
Cheng, SB
Chew, EC
机构
[1] Shantou Univ, Coll Med, Dept Histol & Embryol, Shantou 515031, Guangdong Prov, Peoples R China
[2] Shantou Univ, Coll Med, Tumor Pathol Lab, Shantou 515031, Guangdong Prov, Peoples R China
[3] Chinese Univ Hong Kong, Dept Physiol, Shatin, Hong Kong, Peoples R China
[4] Chinese Univ Hong Kong, Dept Anat, Shatin, Hong Kong, Peoples R China
关键词
D O I
10.3748/wjg.v7.i6.788
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To explore the etiologic role of HPV infection in esophageal carcinoma, and the association of HPV-16 E6 with the nuclear matrix of carcinoma cells. METHODS: Two esophageal carcinoma cell lines, EC/ CUHK1 and EC/CUHK2, were tested for HPV-16 E6 subgenetic fragment by polymerase chain reaction amplification of virus DNA associated nuclear matrix. RT-PCR and immunocytochemistry were also used to visualize the expression of E6 subgene in the cells. RESULTS: The HPV-16 E6 subgenetic fragment was found to be present in nuclear matrix-associated DNA, E6 oncoprotein localized in the nucleus where it is tightly associated with nuclear matrix after sequential extraction in EC/CUHK2 cells. it was not detected, however, in EC/ CUHK1 cells. CONCLUSION: The interaction between HPV-16 E6 and nuclear matrix may contribute to the virus induced carcinogenesis in esophageal carcinoma.
引用
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页码:788 / 791
页数:4
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