Optical biosensing using surface plasmon resonance spectroscopy

The exploitation of surface plasmon resonance (SPR) sensing principle for the study of formation of multilayers of human immunoglobulin (hIgG) and monoclonal antibody against human choriogonadotropin (a-hCG) as well as for monitoring immunoreaction between the immobilized a-hCG and human choriogonadotropin (hCG) is reported. It is demonstrated that even a very small attachment of proteins (due to the adsorption or the specific interaction) may be detected by the presented SPR sensor. An immobilization technique is described which allows to form stable multilayers of antibodies on the gold surface.