Genetic diversity of rhizobia from natural populations varies with the soil dilution sampled

被引：21

作者：

Bala, A

Murphy, P

Giller, KE ^{[1
]}

机构：

[1] Univ London Wye Coll, Dept Biol Sci, Ashford TN25 5AH, Kent, England

[2] Fed Univ Technol, Dept Soil Sci, Minna, Nigeria

[3] Univ Zimbabwe, Dept Soil Sci & Agr Engn, Harare, Zimbabwe

来源：

SOIL BIOLOGY & BIOCHEMISTRY | 2001年 / 33卷 / 06期

关键词：

Calliandra; Leucaena; Gliricidia; PCR-RFLP; most probable number; nodulation; N-2-fixation;

D O I：

10.1016/S0038-0717(00)00221-2

中图分类号：

S15 [土壤学];

学科分类号：

0903 ; 090301 ;

摘要：

Rhizobia were isolated from root nodules of three tree legumes inoculated with dilution series of two tropical soils. The diversity of the isolates obtained from different soil dilutions was assessed by PCR-RFLP analysis of the internally transcribed spacer (ITS) region between the 165 and 23S rRNA genes. In both soils there was a strong effect of dilution on the genetic diversity of the populations with apparent diversity decreasing with increasing dilution. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：841 / 843

页数：3

共 18 条

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[2]

BENNETT JE, 1990, USERS MANUAL MPNES

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