Versatile nitro-fluorophore as highly effective sensor for hypoxic tumor cells: Design, imaging and evaluation

被引:28
作者
Dai, Min
Zhu, Weiping
Xu, Yufang [1 ]
Qian, Xuhong [1 ]
Liu, Yan
Xiao, Yi [2 ]
You, Yin
机构
[1] E China Univ Sci & Technol, Shanghai Key Lab Chem Biol, Sch Pharm, Shanghai 200237, Peoples R China
[2] Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China
基金
中国国家自然科学基金;
关键词
nitro-fluorophore; sensor; hypoxic; tumor cell;
D O I
10.1007/s10895-007-0303-0
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Based on the characteristics of a nitro-group, which can be partially or totally reduced to an amino-group in hypoxic cells, two series of hypoxic sensors containing 7H-Benzimidazole [2,1-a]benz[de]isoquinolin-7-one cores with nitro groups in different positions were designed, synthesized and evaluated. The target compounds exhibited significantly different fluorescence characteristics ( fluorescence enhancement or quenching). The strong fluorescences of some partially reduced compounds could be explained through the examination of their infrared spectra, which showed a restriction of the nitro-group vibration. These compounds exhibited significant hypoxic-oxic fluorescence differences not only in numerical values but also in their fluorescent imaging properties as reported for the first time.
引用
收藏
页码:591 / 597
页数:7
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