Molecular epidemiology and mutations at gyrA and parC genes of ciprofloxacin-resistant Escherichia coli isolates from a Taiwan Medical Center

被引:25
作者
Chen, JY
Siu, LK
Chen, YH
Lu, PL
Ho, M
Peng, CF
机构
[1] Kaohsiung Med Univ Hosp, Dept Internal Med, Kaohsiung, Taiwan
[2] Kaohsiung Med Univ, Sch Technol Med Sci, Kaohsiung, Taiwan
[3] Natl Hlth Res Inst, Div Clin Res, Taipei, Taiwan
来源
MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE | 2001年 / 7卷 / 01期
关键词
D O I
10.1089/107662901750152783
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Sixty-five ciprofloxacin-resistant clinical Escherichia coli isolates were collected from a Taiwan Medical Center from December 1998 to February 1999. All 65 clinical isolates were resistant (MICs greater than or equal to 4 mug/mL) to the following fluoroquinolones: ofloxacin, levofloxacin, sparfloxacin, and trovafloxacin. These isolates were cross-resistant to chloramphenicol (65 isolates, 100%), tetracycline (65 isolates, 100%), cefuroxime (64 isolates, 98.5%), ampicillin (57 isolates, 87.7%), gentamicin (53 isolates, 81.5%), and cephalothin (24 isolates, 36.9%). Pulsed-field gel electrophoresis (PFGE) revealed a high diversity among the genomes of these isolates and indicated that clonal spread was not responsible for the prevalence of ciprofloxacin resistance in the hospital. Sequencing of the polymerase chain reaction (PCR) amplified products of the quinolone resistance determining regions (QRDRs) of gyrA and parC showed that all isolates carrying double mutations in gyrA at codon 83 and 87 and at least one parC mutation at codon 80 and/or 84. The mutation at codon 83 of GyrA from serine to leucine (S83L) was present in all the clinical isolates. The most prevalent pattern was the S83L mutation and the mutation at codon 87 from an aspartate to an asparagine (D87N) of GyrA plus a mutation from a serine to an isoleucine (S80I) at codon 80 of ParC (63.2%). This indicated that the presence of high-level resistance to quinolones in clinical E. coli isolates were associated with mutations at hot spots, codon 83 and 87 in GyrA and followed by subsequent mutation in either codon 80 and/or 84 in ParC.
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页码:47 / 53
页数:7
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共 41 条
  • [1] THE ROLE OF TOPOISOMERASE-IV IN PARTITIONING BACTERIAL REPLICONS AND THE STRUCTURE OF CATENATED INTERMEDIATES IN DNA-REPLICATION
    ADAMS, DE
    SHEKHTMAN, EM
    ZECHIEDRICH, EL
    SCHMID, MB
    COZZARELLI, NR
    [J]. CELL, 1992, 71 (02) : 277 - 288
  • [2] Organic solvent tolerance and antibiotic resistance increased by overexpression of marA in Escherichia coli
    Asako, H
    Nakajima, H
    Kobayashi, K
    Kobayashi, M
    Aono, R
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (04) : 1428 - 1433
  • [3] Analysis of the mutations involved in fluoroquinolone resistance of in vivo and in vitro mutants of Escherichia coli
    Bachoual, R
    Tankovic, J
    Soussy, CJ
    [J]. MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 1998, 4 (04): : 271 - 276
  • [4] Impact of gyrA and parC mutations on quinolone resistance, doubling time, and supercoiling degree of Escherichia coli
    Bagel, S
    Hüllen, V
    Wiedemann, B
    Heisig, P
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (04) : 868 - 875
  • [5] The complete genome sequence of Escherichia coli K-12
    Blattner, FR
    Plunkett, G
    Bloch, CA
    Perna, NT
    Burland, V
    Riley, M
    ColladoVides, J
    Glasner, JD
    Rode, CK
    Mayhew, GF
    Gregor, J
    Davis, NW
    Kirkpatrick, HA
    Goeden, MA
    Rose, DJ
    Mau, B
    Shao, Y
    [J]. SCIENCE, 1997, 277 (5331) : 1453 - +
  • [6] Quinolone resistance locus nfxD of Escherichia coli is a mutant allele of the parE gene encoding a subunit of topoisomerase IV
    Breines, DM
    Ouabdesselam, S
    Ng, EY
    Tankovic, J
    Shah, S
    Soussy, CJ
    Hooper, DC
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1997, 41 (01) : 175 - 179
  • [7] NOVEL GYRA POINT MUTATION IN A STRAIN OF ESCHERICHIA-COLI RESISTANT TO FLUOROQUINOLONES BUT NOT TO NALIDIXIC-ACID
    CAMBAU, E
    BORDON, F
    COLLATZ, E
    GUTMANN, L
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1993, 37 (06) : 1247 - 1252
  • [8] FLUOROQUINOLONE RESISTANCE AMONG METHICILLIN-RESISTANT STAPHYLOCOCCI AFTER USAGE OF FLUOROQUINOLONES OTHER THAN CIPROFLOXACIN IN TAIWAN
    CHANG, SC
    HSIEH, WC
    LUH, KT
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1994, 19 (03) : 143 - 147
  • [9] Antibiotic usage in primary care units in Taiwan
    Chang, SC
    Chang, HJ
    Lai, MS
    [J]. INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS, 1999, 11 (01) : 23 - 30
  • [10] CLONING AND CHARACTERIZATION OF A DNA GYRASE-A GENE FROM ESCHERICHIA-COLI THAT CONFERS CLINICAL RESISTANCE TO 4-QUINOLONES
    CULLEN, ME
    WYKE, AW
    KURODA, R
    FISHER, LM
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1989, 33 (06) : 886 - 894