Application and evaluation of a new cold-stable kinetic Jaffe reagent to the Hitachi 747 for the determination of serum creatinine

century the most commonly used reagents have been those based upon the work of Jaffe [1], which are composed of alkali and picric acid. Upon addition of creatinine to this reagent a red color, referred to as a Janovsky complex [2,3] is rapidly formed. Almost all of the currently available approaches to the automated determination of creatinine are based upon this reaction. Furthermore, the rate of the reaction may be controlled so that either equilibrium [4] or kinetic [5] measurements can be made. Although various enzymic approaches have been proposed [6-10] they, as yet, have not gained the same popular support of the modified Jaffe reagents. (C) 2000 Elsevier Science B.V. All rights reserved.