Species identification and strain differentiation of dermatophyte fungi by analysis of ribosomal-DNA intergenic spacer regions

被引:171
作者
Jackson, CJ
Barton, RC
Evans, EGV
机构
[1] Univ Leeds, Old Med Sch, Dept Microbiol, PHLS Mycol Reference Lab, Leeds LS2 9JT, W Yorkshire, England
[2] Gen Infirm, Leeds LS2 9JT, W Yorkshire, England
关键词
D O I
10.1128/JCM.37.4.931-936.1999
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Restriction fragment length polymorphisms (RFLPs) identified in the ribosomal-DNA (rDNA) repeat were used for molecular strain differentiation of the dermatophyte fungus Trichophyton rubrum. The polymorphisms were detected by hybridization of EcoRI-digested T. rubrum genomic DNAs with a probe amplified from the small-subunit (18S) rDNA and adjacent internal transcribed spacer (TTS) regions. The rDNA RFLPs mapped to the nontranscribed spacer (NTS) region of the rDNA repeat and appeared similar to those caused by short repetitive sequences in the intergenic spacers of other fungi. Fourteen individual RFLP patterns (DNA types A to N) were recognized among 50 random clinical isolates of T. rubrum, A majority of strains (19 of 50 [38%]) were characterized by one RFLP pattern (DNA type A), and four types (DNA types A to D) accounted for 78% (39 of 50) of all strains. The remaining types (DNA types E to N) were represented by one or two isolates only. A rapid and simple method was also developed for molecular species identification of dermatophyte fungi. The contiguous ITS and 5.8S rDNA regions were amplified from 17 common dermatophyte species by using the universal primers ITS 1 and ITS 4. Digestion of the amplified ITS products with the restriction endonuclease MvaI produced unique and easily identifiable fragment patterns for a majority of species. However, some closely related taxon pairs, such as T. rubrum-T. soudanense and T. quinkeanum-T. schoenlenii could not be distinguished. We conclude that RFLP analysis of the NTS and ITS intergenic regions of the rDNA repeat is a valuable technique both for molecular strain differentiation of T. rubrum and for species identification of common dermatophyte fungi.
引用
收藏
页码:931 / 936
页数:6
相关论文
共 27 条
[1]   IS PENICILLIUM MONOPHYLETIC - AN EVALUATION OF PHYLOGENY IN THE FAMILY TRICHOCOMACEAE FROM 18S, 5.8S AND ITS RIBOSOMAL DNA-SEQUENCE DATA [J].
BERBEE, ML ;
YOSHIMURA, A ;
SUGIYAMA, J ;
TAYLOR, JW .
MYCOLOGIA, 1995, 87 (02) :210-222
[2]  
BOWMAN BH, 1992, MOL BIOL EVOL, V9, P893
[3]   Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1 [J].
Carlotti, A ;
Chaib, F ;
Couble, A ;
Bourgeois, N ;
Blanchard, V ;
Villard, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (06) :1337-1343
[4]   A novel repeat sequence (CKRS-1) containing a tandemly repeated sub-element (kre) accounts for differences between Candida krusei strains fingerprinted with the probe CkF1,2 [J].
Carlotti, A ;
Srikantha, T ;
Schroppel, K ;
Kvaal, C ;
Villard, J ;
Soll, DR .
CURRENT GENETICS, 1997, 31 (03) :255-263
[5]   Causative pathogens in onychomycosis and the possibility of treatment resistance: A review [J].
Evans, EGV .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1998, 38 (05) :S32-S36
[6]   Increasing frequency of onychomycoses - Is there a change in the spectrum of infectious agents? [J].
Ginter, G ;
Rieger, E ;
Heigl, K ;
Propst, E .
MYCOSES, 1996, 39 :118-122
[7]  
Gräser Y, 1998, BRIT J DERMATOL, V138, P576, DOI 10.1046/j.1365-2133.1998.02165.x
[8]  
Kane J, 1997, LAB HDB DERMATOPHYTE, P45
[9]   SUBREPEAT STRUCTURE OF THE INTERGENIC REGION IN THE RIBOSOMAL DNA OF THE OOMYCETOUS FUNGUS PYTHIUM-ULTIMUM [J].
KLASSEN, GR ;
BUCHKO, J .
CURRENT GENETICS, 1990, 17 (02) :125-127
[10]  
LECLERC MC, 1994, J MED VET MYCOL, V32, P331