Flagellin gene and protein variation amongst clinical isolates of Pseudomonas aeruginosa

被引:42
作者
Winstanley, C
Coulson, MA
Wepner, B
Morgan, JAW
Hart, CA
机构
[1] HORT RES INT,WELLESBOURNE CV35 9EF,WARWICK,ENGLAND
[2] ROYAL LIVERPOOL UNIV HOSP,DEPT MED MICROBIOL,LIVERPOOL L7 8XP,MERSEYSIDE,ENGLAND
来源
MICROBIOLOGY-UK | 1996年 / 142卷
关键词
Pseudomonas aeruginosa; flagellin; PCR; genetic diversity; typing;
D O I
10.1099/13500872-142-8-2145
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Flagellin gene sequences from 64 clinical isolates of the opportunistic pathogen Pseudomonas aeruginosa were amplified by PCR and subjected to RFLP analysis by using seven restriction enzymes to digest the amplified products. Using this approach the isolates were assigned to one of 13 groups. The method was rapid, reproducible and applicable to all isolates. In contrast, serotyping failed to satisfactorily resolve 49% of the strains tested. The vast majority of clinical isolates generated amplified products of 1.02 kb (type a) or 1.25 kb (type b). Electron microscopical analysis revealed evidence for some flagellar structural variation between P. aeruginosa strains. This study provides further evidence that the flagellin gene is a widely applicable and useful genetic marker for studying genetic variation within populations of closely related bacteria.
引用
收藏
页码:2145 / 2151
页数:7
相关论文
共 25 条
[1]   ELECTROPHORETIC SEPARATION AND MOLECULAR-WEIGHT CHARACTERIZATION OF PSEUDOMONAS-AERUGINOSA H-ANTIGEN FLAGELLINS [J].
ALLISON, JS ;
DAWSON, M ;
DRAKE, D ;
MONTIE, TC .
INFECTION AND IMMUNITY, 1985, 49 (03) :770-774
[2]   DISTRIBUTION AND POLYMORPHISM OF THE FLAGELLIN GENES FROM ISOLATES OF CAMPYLOBACTER-COLI AND CAMPYLOBACTER-JEJUNI [J].
ALM, RA ;
GUERRY, P ;
TRUST, TJ .
JOURNAL OF BACTERIOLOGY, 1993, 175 (10) :3051-3057
[3]  
BODEY GP, 1983, REV INFECT DIS, V5, P279
[4]   ISOLATION AND CHARACTERIZATION OF PSEUDOMONAS-PSEUDOMALLEI FLAGELLIN PROTEINS [J].
BRETT, PJ ;
MAH, DCW ;
WOODS, DE .
INFECTION AND IMMUNITY, 1994, 62 (05) :1914-1919
[5]  
DRAKE D, 1988, J GEN MICROBIOL, V134, P43
[6]   PROTECTION AGAINST PSEUDOMONAS-AERUGINOSA INFECTION BY PASSIVE TRANSFER OF ANTI-FLAGELLAR SERUM [J].
DRAKE, D ;
MONTIE, TC .
CANADIAN JOURNAL OF MICROBIOLOGY, 1987, 33 (09) :755-763
[7]   PSEUDOMONAS-AERUGINOSA SEROTYPE O-12 OUTBREAK STUDIED BY ARBITRARY PRIMER PCR [J].
ELAICHOUNI, A ;
VERSCHRAEGEN, G ;
CLAEYS, G ;
DEVLEESCHOUWER, M ;
GODARD, C ;
VANEECHOUTTE, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (03) :666-671
[8]   DIFFERENTIATION OF PSEUDOMONAS-AERUGINOSA STRAINS BY RIBOTYPING - HIGH DISCRIMINATORY POWER BY USING A SINGLE RESTRICTION-ENDONUCLEASE [J].
GRATTARD, F ;
POZZETTO, B ;
ROS, A ;
GAUDIN, OG .
JOURNAL OF MEDICAL MICROBIOLOGY, 1994, 40 (04) :275-281
[9]   DISCRIMINATORY POWER OF 3 DNA-BASED TYPING TECHNIQUES FOR PSEUDOMONAS-AERUGINOSA [J].
GRUNDMANN, H ;
SCHNEIDER, C ;
HARTUNG, D ;
DASCHNER, FD ;
PITT, TL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) :528-534
[10]  
GRUNDMANN HJ, 1994, MED MICROBIOL LETT, V3, P42