RP-HPLC for the determination of N-phenylhydroxylamine and related compounds

被引：3

作者：

Stolcová, M ^{[1
]}

Grosková, D ^{[1
]}

Hronec, M ^{[1
]}

机构：

[1] Slovak Tech Univ Bratislava, Fac Chem Technol, Dept Organ Technol, Bratislava 81237, Slovakia

来源：

CHROMATOGRAPHIA | 2001年 / 53卷 / Suppl 1期

关键词：

column liquid chromatography; N-phenylhydroxylamine metabolites; nitrogen-containing compounds;

D O I：

10.1007/BF02490377

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple, reversed-phase high performance liquid chromatographic method for the simultaneous determination of N-phenylhydroxylamine and related nitrogen-containing compounds, without poor peak asymmetries, is described. The analytes were detected at different wavelength depending on their absorbance maxima using a diode array detector after separation on an octadecyl-bonded silica, Inertsil ODS-2, with aqueous potassium citrate buffered (pH 7) mobile phase modified with methanol as organic solvent under gradient elution. The determined limits of quantification, measuring peak areas relative to an internal standard (N-tertbutyl-2-benzothiazolesulphenamide), are in the range of ng.mL(-1).

引用

页码：S456 / S459

页数：4

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