Simultaneous two-photon imaging and photo-stimulation with structured light illumination

被引：61

作者：

Dal Maschio, Marco ^{[1
]}

Difato, Francesco ^{[1
]}

Beltramo, Riccardo ^{[1
]}

Blau, Axel ^{[1
]}

Benfenati, Fabio ^{[1
]}

Fellin, Tommaso ^{[1
]}

机构：

[1] Italian Inst Technol, Dept Neurosci & Brain Technol, I-16163 Genoa, Italy

来源：

OPTICS EXPRESS | 2010年 / 18卷 / 18期

关键词：

HOLOGRAPHIC OPTICAL TWEEZERS; FLUORESCENCE MICROSCOPY; ABERRATION CORRECTION; MODULATORS;

D O I：

10.1364/OE.18.018720

中图分类号：

O43 [光学];

学科分类号：

070207 [光学];

摘要：

Holographic microscopy is increasingly recognized as a promising tool for the study of the central nervous system. Here we present a "holographic module", a simple optical path that can be combined with commercial scanheads for simultaneous imaging and uncaging with structured two-photon light. The present microscope is coupled to two independently tunable lasers and has two principal configurations: holographic imaging combined with galvo-steered uncaging and holographic uncaging combined with conventional scanning imaging. We applied this flexible system for simultaneous two-photon imaging and photostimulation of neuronal cells with complex light patterns, opening new perspectives for the study of brain function in situ and in vivo. (C) 2010 Optical Society of America

引用

页码：18720 / 18731

页数：12

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