A convenient and reproducible method to genetically transform bacteria of the genus Bifidobacterium

被引：54

作者：

Argnani, A ^{[1
]}

Leer, RJ ^{[1
]}

vanLuijk, N ^{[1
]}

Pouwels, PH ^{[1
]}

机构：

[1] TNO,NUTR & FOOD RES INST,DEPT MOLEC GENET & GENE TECHNOL,2280 HV RIJSWIJK,NETHERLANDS

来源：

MICROBIOLOGY-UK | 1996年 / 142卷

关键词：

Bifidobacterium; electrotransformation; plasmid vector;

D O I：

10.1099/13500872-142-1-109

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A protocol was developed for the introduction of foreign plasmid DNA into various Bifidobacterium strains. The method, which is applicable to all Bifidobacterium species tested so far, is based on electroporation of bacteria made competent by preincubation in electroporation buffer for several hours at 4 degrees C. Transformation of Bifidobacterium could be achieved with a plasmid vector originating from Bifidobacterium and with plasmid vectors from Corynebacterium, but not with vectors carrying replicons from Lactococcus or Lactobacillus.

引用

页码：109 / 114

页数：6

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