Evidence that anti-type VII collagen antibodies are pathogenic and responsible for the clinical, histological, and immunological features of epidermolysis bullosa acquisita

被引:88
作者
Woodley, DT
Chang, C
Saadat, P
Ram, R
Liu, Z
Chen, M
机构
[1] Univ So Calif, Keck Sch Med, Div Dermatol, Dept Med, Los Angeles, CA 90033 USA
[2] Univ N Carolina, Dept Dermatol, Chapel Hill, NC 27514 USA
关键词
animal model; autoimmunity; basement membrane; bullous disease; skin; type VII collagen;
D O I
10.1111/j.0022-202X.2005.23702.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Epidermolysis bullosa acquisita (EBA) is an autoimmune blistering disease characterized by autoantibodies to type VII (anchoring fibril) collagen. Therefore, it is a prototypic autoimmune disease defined by a well-known autoantigen and autoantibody. In this study, we injected hairless immune competent mice with purified immunoglobulin G (IgG) fraction of serum from rabbits immunized with the non-collagenous amino-terminal domain (NC1) of human type VII collagen, the domain known to contain immunodominant epitopes. As a control, identical mice were injected with the IgG fraction of serum from non-immunized rabbits. Mice injected with immune IgG developed subepidermal skin blisters and erosions, IgG deposits at the epidermal-dermal junction of their skin, and circulating anti-NC1 antibodies in their serum-all features reminiscent of patients with EBA. Similar concentrations of control IgG purified from normal rabbits did not induce disease in the mice. These findings strongly suggest that autoantibodies that recognize human type VII collagen in EBA are pathogenic. This murine model, with features similar to the clinical, histological, and immunological features of EBA, will be useful for the fine dissection of immunopathogenic mechanisms in EBA and for the development of new therapeutic interventions.
引用
收藏
页码:958 / 964
页数:7
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