ZAP-70 is expressed by a subset of normal human B-lymphocytes displaying an activated phenotype

被引:70
作者
Nolz, JC
Tschumper, RC
Pittner, BT
Darce, JR
Kay, NE
Jelinek, DF
机构
[1] Mayo Clin, Coll Med, Mayo Grad Sch, Dept Immunol, Rochester, MN 55905 USA
[2] Mayo Clin, Coll Med, Mayo Grad Sch, Dept Internal Med,Div Hematol, Rochester, MN USA
关键词
ZAP-70; B-CLL; B-lymphocytes; lymphocyte activation;
D O I
10.1038/sj.leu.2403726
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The Syk family tyrosine kinase ZAP-70 is essential for normal T-cell development and signaling. Recently, leukemic cells from some patients with B-cell chronic lymphocytic leukemia (B-CLL) were shown to express ZAP-70. Owing to the prognostic value of B-CLL ZAP-70 expression, this phenotype may reflect intrinsic biological differences between the two subsets of disease. However, it remains unclear whether CLL-B cells aberrantly acquire ZAP-70 expression during the transformation process or whether ZAP-70 may be expressed under certain conditions in normal human B-lymphocytes. To discriminate between these two possibilities, we assessed ZAP-70 expression in normal human B-lymphocytes. Our data demonstrate that ZAP-70 is expressed in a subpopulation of tonsillar and splenic normal B-lymphocytes that express an activated phenotype. Furthermore, ZAP-70 expression can be induced in vitro upon stimulation of blood and tonsillar B cells. Finally, we show that phosphorylation of ZAP-70 occurs in tonsillar B cells with stimulation through the B-cell receptor. These results provide new insight into normal human B-cell biology as well as provide clues about the transformed cell in B-CLL.
引用
收藏
页码:1018 / 1024
页数:7
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