Age-related persistent clonal expansions of CD28- cells:: Phenotypic and molecular TCR analysis reveals both CD4+ and CD4+ CD8+ cells with identical CDR3 sequences

被引:50
作者
Colombatti, A [1 ]
Doliana, R
Schiappacassi, M
Argentini, C
Tonutti, E
Feruglio, C
Sala, P
机构
[1] Ctr Riferimento Oncol, Div Oncol Sperimentale 2, I-33081 Aviano, Italy
[2] Univ Udine, Dipartimento Sci & Tecnol Biomed, I-33100 Udine, Italy
[3] Ospedale S Maria Misericordia, Ist Chim Clin, Udine, Italy
来源
CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1998年 / 89卷 / 01期
关键词
clonal expansion; elderly; CD28(-) cells; CD4(+) cells; CD4(+)CD8(+) cells;
D O I
10.1006/clin.1998.4580
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In a small group of subjects we had identified persistent expansions (range 6-72%) of CD4(+)CD8(+) double-positive (DP) peripheral blood (PB) cells which express the CDS alpha/alpha homodimer. Here, DP cells present in a larger cohort were further investigated and found by FACS analysis to express a single or a dominant TCRBV family. In these subjects, with a mean age of about 64 years, expansions of CD4(+) cells with the same TCRBV family specificity as in the respective DP cells also were consistently detected. TCR heterogeneity of the dominant TCRBV family was specifically evaluated: The amplified CDR3 region was cloned and found to consist of one single or two largely dominant sequence patterns. Furthermore, cloning of the CDR3 region from FACS-sorted DP, CD4(+), or CD8(+) cells indicates that both DP and CD4(+), but not CD8(+) cells, isolated from the same individual possess a striking identity of the CDR3 regions. As indicated by FACS analysis, the clonally expanded cells occur in the CD4(+)CD28(-) cells. Taken together, these results suggest that expanded CD4(+)CD28(-) cells might also acquire CD8 alpha/alpha expression and become DP and imply that CD4 clonality is a more frequent phenomenon than previously suspected. In conclusion, the persistent expansions described in this report represent a novel group of age-related benign clonal expansions of still undefined significance of a rare CD28(-) T cell subset. (C) 1998 Academic Press.
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页码:61 / 70
页数:10
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