General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin

被引：80

作者：

Verloo, D ^{[1
]}

Magnus, E ^{[1
]}

Büscher, P ^{[1
]}

机构：

[1] Inst Trop Med, Dept Parasitol, B-2000 Antwerp, Belgium

来源：

VETERINARY PARASITOLOGY | 2001年 / 97卷 / 03期

关键词：

Trypanosoma evansi; diagnosis-protozoa; serology; rabbit; experimental;

D O I：

10.1016/S0304-4017(01)00412-5

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The variable surface glycoprotein of Trypanosoma evansi RoTat 1.2 variable antigen type (VAT) is used as an antigen in different antibody detection assays for T. evansi. To obtain more information on the predominant character of RoTat 1.2 and its diagnostic potential in antibody detection tests, we checked its expression in 10 different T. evansi stocks and clones from different parts of the world. Cryostabilates were injected into mice and the trypanosomes of the first peak parasitaemia were screened for the presence of RoTat 1.2 by VAT specific immunofluorescence. To monitor the appearance of RoTat 1.2 specific antibodies during infection, rabbits were infected and serologically tested at different time intervals with VAT specific immune trypanolysis, CATT/T. evansi, LATEX/T. evansi and ELISA/T. evansi. Test results confirm the predominant character of RoTat 1.2. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：183 / 189

页数：7

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