Optimisation of sample preparation for the determination of trans-resveratrol and other polyphenolic compounds in wines by high performance liquid chromatography

被引：106

作者：

Malovaná, S

Montelongo, FJG

Pérez, JP

Rodríguez-Delgado, MA ^{[1
]}

机构：

[1] Univ La Laguna, Dept Analyt Chem, Tenerife 38071, Spain

[2] Masaryk Univ, Fac Sci, Dept Analyt Chem, CZ-61137 Brno, Czech Republic

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 428卷 / 02期

关键词：

sample preparation; trans-resveratrol; phenolic compounds; HPLC; wine samples;

D O I：

10.1016/S0003-2670(00)01231-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Different methods for sample preparation as a preliminary stage for the simultaneous determination of trans-resveratrol and other polyphenolic compounds are compared with the aim to establish the best conditions for the determination of these compounds in wine samples by high performance liquid chromatography (HPLC). Sample preparation using liquid-liquid extraction and solid-phase extraction were compared. Liquid-liquid extraction using as organic solvent diethyl ether at pH 2.0 shows the best results. The dried extract was solved in methanol/water 1:1 (v/v) that improve drastically the separation efficiency. The developed method was applied to the analysis of wines from the Canary Islands. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：245 / 253

页数：9

共 27 条

[1] Determination of trans-resveratrol and other polyphenols in wines by a continuous flow sample clean-up system followed by capillary electrophoresis separation [J].