Determination of creatinine in clinical samples based on flow-through microsystem

被引：12

作者：

Grabowska, I ^{[1
]}

Chudy, M ^{[1
]}

Dybko, A ^{[1
]}

Brzozka, Z ^{[1
]}

机构：

[1] Warsaw Univ Technol, Dept Analyt Chem, PL-00664 Warsaw, Poland

来源：

ANALYTICA CHIMICA ACTA | 2005年 / 540卷 / 01期

关键词：

creatinine determination; flow-through microsystem; PDMS; Jaffe reaction; optical detection;

D O I：

10.1016/j.aca.2004.09.021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The paper describes a flow-through microsystem with optical detection for measurement of creatinine concentration in urine and post-dialysate fluid. The microsystem is made of a transparent polymer-poly(dimethyliosiloxane) (PDMS). In order to determine creatinine, classical Jaffe reaction was carried out. The influence of reaction parameters (temperature and solutions flow rate) on its sensitivity and repeatability of recorded signal was investigated. Results of creatinine determination in postdialysate fluids and urine were compared to results obtained in hospital laboratories. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：181 / 185

页数：5

共 14 条

[1]

[Anonymous], 1986, TXB CLIN CHEM

[2] Analysis of creatine and creatinine in urine by capillary electrophoresis [J].