机构:
Univ Chicago, Comm Genet, Chicago, IL 60637 USA
Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USAUniv Chicago, Comm Genet, Chicago, IL 60637 USA
McMahill, Melissa S.
[1
,2
]
Sham, Caroline W.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USAUniv Chicago, Comm Genet, Chicago, IL 60637 USA
Sham, Caroline W.
[2
]
Bishop, Douglas K.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Chicago, Comm Genet, Chicago, IL 60637 USA
Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA
Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USAUniv Chicago, Comm Genet, Chicago, IL 60637 USA
Bishop, Douglas K.
[1
,2
,3
]
机构:
[1] Univ Chicago, Comm Genet, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA
[3] Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
来源:
PLOS BIOLOGY
|
2007年
/
5卷
/
11期
关键词:
D O I:
10.1371/journal.pbio.0050299
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and noncrossover recombinants (NCOs); however, dHJs are now thought to give rise mainly to COs, with SDSA forming most or all NCOs. To test this model in Saccharomyces cerevisiae, we constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA, but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double-strand break site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This diagnostic class represents 26% of selected NCO recombinants. Tetrad analysis using the same markers provided additional evidence that SDSA is a major pathway for NCO gene conversion in meiosis.