Synthesis-dependent strand annealing in meiosis

被引:111
作者
McMahill, Melissa S. [1 ,2 ]
Sham, Caroline W. [2 ]
Bishop, Douglas K. [1 ,2 ,3 ]
机构
[1] Univ Chicago, Comm Genet, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Radiat & Cellular Oncol, Chicago, IL 60637 USA
[3] Univ Chicago, Dept Mol Genet & Cell Biol, Chicago, IL 60637 USA
来源
PLOS BIOLOGY | 2007年 / 5卷 / 11期
关键词
D O I
10.1371/journal.pbio.0050299
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Recent studies led to the proposal that meiotic gene conversion can result after transient engagement of the donor chromatid and subsequent DNA synthesis-dependent strand annealing (SDSA). Double Holliday junction (dHJ) intermediates were previously proposed to form both reciprocal crossover recombinants (COs) and noncrossover recombinants (NCOs); however, dHJs are now thought to give rise mainly to COs, with SDSA forming most or all NCOs. To test this model in Saccharomyces cerevisiae, we constructed a random spore system in which it is possible to identify a subset of NCO recombinants that can readily be accounted for by SDSA, but not by dHJ-mediated recombination. The diagnostic class of recombinants is one in which two markers on opposite sides of a double-strand break site are converted, without conversion of an intervening heterologous insertion located on the donor chromatid. This diagnostic class represents 26% of selected NCO recombinants. Tetrad analysis using the same markers provided additional evidence that SDSA is a major pathway for NCO gene conversion in meiosis.
引用
收藏
页码:2589 / 2601
页数:13
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