Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses
被引:241
作者:
Nicholls, John M.
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机构:
Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
Nicholls, John M.
[1
]
Bourne, Anthony J.
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机构:
Adelaide Women & Childrens Hosp, Dept Pathol, Adelaide, SA 5006, AustraliaUniv Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
Bourne, Anthony J.
[2
]
Chen, Honglin
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机构:
Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
Chen, Honglin
[3
]
Guan, Yi
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机构:
Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
Guan, Yi
[3
]
Peiris, J. S. Malik
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机构:
Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R ChinaUniv Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
Peiris, J. S. Malik
[3
]
机构:
[1] Univ Hong Kong, Dept Pathol, Hong Kong, Hong Kong, Peoples R China
[2] Adelaide Women & Childrens Hosp, Dept Pathol, Adelaide, SA 5006, Australia
[3] Univ Hong Kong, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China
来源:
RESPIRATORY RESEARCH
|
2007年
/
8卷
/
1期
基金:
英国惠康基金;
关键词:
D O I:
10.1186/1465-9921-8-73
中图分类号:
R56 [呼吸系及胸部疾病];
学科分类号:
摘要:
Background: Influenza virus binds to cell receptors via sialic acid ( SA) linked glycoproteins. They recognize SA on host cells through their haemagglutinins (H). The distribution of SA on cell surfaces is one determinant of host tropism and understanding its expression on human cells and tissues is important for understanding influenza pathogenesis. The objective of this study therefore was to optimize the detection of alpha 2,3-linked and alpha 2,6-linked SA by lectin histochemistry by investigating the binding of Sambucus nigra agglutinin (SNA) for SA alpha 2,6Gal and Maackia amurensis agglutinin (MAA) for SA alpha 2,3Gal in the respiratory tract of normal adults and children. Methods: We used fluorescent and biotinylated SNA and MAA from different suppliers on archived and prospectively collected biopsy and autopsy specimens from the nasopharynx, trachea, bronchus and lungs of fetuses, infants and adults. We compared different methods of unmasking for tissue sections to determine if these would affect lectin binding. Using serial sections we then compared the lectin binding of MAA from different suppliers. Results: We found that unmasking using microwave treatment in citrate buffer produced increased lectin binding to the ciliated and glandular epithelium of the respiratory tract. In addition we found that there were differences in tissue distribution of the alpha 2,3 linked SA when 2 different isoforms of MAA (MAA1 and MAA2) lectin were used. MAA1 had widespread binding throughout the upper and lower respiratory tract and showed more binding to the respiratory epithelium of children than in adults. By comparison, MAA2 binding was mainly restricted to the alveolar epithelial cells of the lung with weak binding to goblet cells. SNA binding was detected in bronchial and alveolar epithelial cells and binding of this lectin was stronger to the paediatric epithelium compared to adult epithelium. Furthermore, the MAA lectins from 2 suppliers (Roche and EY Labs) tended to only bind in a pattern similar to MAA1 (Vector Labs) and produced a different binding pattern to MAA2 from Vector Labs. Conclusion: The lectin binding pattern of MAA may vary depending on the supplier and the different isoforms of MAA show a different tissue distribution in the respiratory tract. This finding is important if conclusions about the potential binding sites of SA alpha 2,3 binding viruses, such as influenza or human parainfluenza are to be made.
机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Gagneux, P
Cheriyan, M
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Cheriyan, M
Hurtado-Ziola, N
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Hurtado-Ziola, N
van der Linden, ECMB
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
van der Linden, ECMB
Anderson, D
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Anderson, D
McClure, H
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
McClure, H
Varki, A
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Varki, A
Varki, NM
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机构:
Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Gagneux, P
Cheriyan, M
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h-index: 0
机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Cheriyan, M
Hurtado-Ziola, N
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h-index: 0
机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Hurtado-Ziola, N
van der Linden, ECMB
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
van der Linden, ECMB
Anderson, D
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Anderson, D
McClure, H
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
McClure, H
Varki, A
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机构:Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA
Varki, A
Varki, NM
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机构:
Univ Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USAUniv Calif San Diego, Dept Med, Glyocobiol Res & Training Ctr, La Jolla, CA 92093 USA