Cross-linked LDH crystals for lactate synthesis coupled to electroenzymatic regeneration of NADH

被引：41

作者：

Sobolov, SB ^{[1
]}

Leonida, MD ^{[1
]}

BartoszkoMalik, A ^{[1
]}

Voivodov, KI ^{[1
]}

McKinney, F ^{[1
]}

Kim, J ^{[1
]}

Fry, AJ ^{[1
]}

机构：

[1] WESLEYAN UNIV, DEPT CHEM, MIDDLETOWN, CT 06459 USA

来源：

JOURNAL OF ORGANIC CHEMISTRY | 1996年 / 61卷 / 06期

关键词：

D O I：

10.1021/jo951408v

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Lactate dehydrogenase (LDH) was crystallized from concentrated ammonium sulfate solution and cross-linked with glutaraldehyde to afford long-lived enzymatically active cross-linked crystals (LDH-CLC). The crystals were employed in an electrolytic cell for lactate production from pyruvate, in which the cathode consisted of a carbon electrode containing a coating of lipoamide dehydrogenase (LiDH) immobilized with methyl viologen under a Nafion membrane. This cell was more effective than a similar cell containing LDH in soluble form. An even greater improvement in performance was achieved by chemically binding a viologen derivative to the LiDH and using an electrode based on this modified enzyme in a cell containing LDH-CLC. The activity of the LDH-CLC is much less sensitive to pH than that of the soluble enzyme.

引用

页码：2125 / 2128

页数：4

共 23 条

[1] DIRECT ELECTROCHEMISTRY OF REDOX PROTEINS [J].