Surface plasmon resonance and impedance spectroscopy on gold electrode for biosensor application

被引：16

作者：

Helali, S. ^{[2
]}

Ben Fredj, H. ^{[2
]}

Cherif, K. ^{[2
]}

Abdelghani, A. ^{[2
]}

Martelet, C. ^{[1
]}

Jaffrezic-Renault, N. ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, Sci Analyt Lab, UMR 5180, F-69622 Villeurbanne, France

[2] IPEST, Unite Rech Phys Semicond & Capteurs, La Marsa 2070, Tunisia

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 5-6期

关键词：

self-assembled multilayer; surface plasmon resonance; electrochemical impedance spectroscopy; biosensor;

D O I：

10.1016/j.msec.2007.10.065

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

This paper describes new-label free immunoassays for the detection of atrazine using optical Surface Plasmon Resonance (SPR) and Electrochemical Impedance Spectroscopy (EIS) by employing gold functionalised with self-assembled mixed monolayer. The mixed monolayer, formed by 16-mercaptohexadecanoic acid (MHDA) and biotinyl-PE, is used as the basic layer in the fabrication of the immunoprobe. The interfacial interaction of a biotinyl-Fab fragment K47 antibody with the mixed monolayer is based on a biotin-avidin system using the neutravidin molecule. Thus, in this study the multilayer engineering is mentored by SPR and EIS methods. In addition, the specificity and sensitivity of this self-assembled multilayer system to the presence of atrazine are investigated. The limit detection of Atrazine obtained with EIS technique is equal to 20 ng/ml and with SPR technique is equal to 50 ng/ml. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：588 / 593

页数：6

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