Acquisition of multiple image stacks with a confocal laser scanning microscope

被引：12

作者：

Zuschratter, W ^{[1
]}

Steffen, T ^{[1
]}

Braun, K ^{[1
]}

Herzog, A ^{[1
]}

Michaelis, B ^{[1
]}

Scheich, H ^{[1
]}

机构：

[1] Fed Inst Neurobiol, D-39118 Magdeburg, Germany

来源：

THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING V, PROCEEDINGS OF | 1998年 / 3261卷

关键词：

multi-image-stacks; confocal laserscanning microscopy; 3D image acquisition;

D O I：

10.1117/12.310551

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Image acquisition at high magnification is inevitably correlated with a limited view over the entire tissue section. To overcome this limitation we designed software for multiple image-stack acquisition (3D-MISA) in confocal laser scanning microscopy (CLSM). The system consists of a 4 channel Leica CLSM equipped with a high resolution z-scanning stage (Leica Lasertechnik) mounted on a xy-motorized stage (Marzhauser). The 3D-MISA software is implemented into the microscope scanning software (Scanware, Leica Lasertechnik) and uses the microscope settings (magnification, zoom) for the movements of the xy-stage. It allows storage and recall of 70 xyz-positions and the automatic 3D-scanning of image arrays between selected xyz-coordinates. The number of images within one array is limited only by the amount of disk space or memory available. Although for most applications the accuracy of the xy-scanning stage is sufficient for a precise alignment of tiled views, the software provides the possibility of an adjustable overlap between two image stacks by shifting the moving steps of the xy-scanning stage. After scanning a tiled image gallery of the extended focus-images of each channel will be displayed on a graphic monitor. In addition, a tiled image gallery of individual focal planes can be created. In summary, the 3D-MISA allows 3D-image acquisition of coherent regions in combination with high resolution of single images.

引用

页码：177 / 186

页数：10