Evaluation of a colorimetric PCR-based assay to diagnose Plasmodium falciparum malaria in travelers

被引：34

作者：

Zhong, KJY

Kain, KC

机构：

[1] Toronto Hosp, Trop Dis Unit, Dept Med, Div Infect Dis, Toronto, ON M5G 2C4, Canada

[2] Univ Toronto, Toronto, ON, Canada

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 02期

关键词：

D O I：

10.1128/JCM.37.2.339-341.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

New diagnostic tests are needed to facilitate the diagnosis of Plasmodium falciparum malaria in the returned traveler. We performed a blinded evaluation of a nonisotopic colorimetric PCR-based assay (Digene SHARP Signal System) and compared the results with those obtained by microscopy and nested PCR for the detection of P. falciparum malaria in 150 febrile travelers. By using nested PCR as the reference standard, the colorimetric assay had a sensitivity of 100% and a specificity of 95.4% far the detection of P. falciparum, This PCR-based nonisotopic assay is a rapid, sensitive, and specific method for the detection of falciparum malaria in returned travelers.

引用

页码：339 / 341

页数：3

共 14 条

[1] DEMONSTRATION BY THE POLYMERASE CHAIN-REACTION OF MIXED PLASMODIUM-FALCIPARUM AND PLASMODIUM-VIVAX INFECTIONS UNDETECTED BY CONVENTIONAL MICROSCOPY
BROWN, AE
KAIN, KC
PIPITHKUL, J
WEBSTER, HK
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1992, 86 (06) : 609 - 612
[2] RATES OF FORMATION AND THERMAL STABILITIES OF RNA-DNA AND DNA-DNA DUPLEXES AT HIGH-CONCENTRATIONS OF FORMAMIDE
CASEY, J
DAVIDSON, N
[J]. NUCLEIC ACIDS RESEARCH, 1977, 4 (05) : 1539 - 1552
[3] ParaSight(R)F test compared with the polymerase chain reaction and microscopy for the diagnosis of Plasmodium falciparum malaria in travelers
Humar, A
Ohrt, C
Harrington, MA
Pillai, D
Kain, KC
[J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1997, 56 (01) : 44 - 48
[4] Evaluation of a non-isotopic polymerase chain reaction-based assay to detect and predict treatment failure of Plasmodium vivax malaria in travellers
Humar, A
Harrington, MA
Kain, KC
[J]. TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1997, 91 (04) : 406 - 409
[5] Imported malaria: Prospective analysis of problems in diagnosis and management
Kain, KC
Harrington, MA
Tennyson, S
Keystone, JS
[J]. CLINICAL INFECTIOUS DISEASES, 1998, 27 (01) : 142 - 149
[6] QUALITATIVE AND SEMIQUANTITATIVE POLYMERASE CHAIN-REACTION TO PREDICT PLASMODIUM-FALCIPARUM TREATMENT FAILURE
KAIN, KC
KYLE, DE
WONGSRICHANALAI, C
BROWN, AE
WEBSTER, HK
VANIJANONTA, S
LOOAREESUWAN, S
[J]. JOURNAL OF INFECTIOUS DISEASES, 1994, 170 (06) : 1626 - 1630
[7] DETECTION OF PLASMODIUM-VIVAX BY POLYMERASE CHAIN-REACTION IN A FIELD-STUDY
KAIN, KC
BROWN, AE
MIRABELLI, L
WEBSTER, HK
[J]. JOURNAL OF INFECTIOUS DISEASES, 1993, 168 (05) : 1323 - 1326
[8] AVOIDING FALSE POSITIVES WITH PCR
KWOK, S
HIGUCHI, R
[J]. NATURE, 1989, 339 (6221) : 237 - 238
[9] PLASMODIUM - GENUS-CONSERVED PRIMERS FOR SPECIES IDENTIFICATION AND QUANTITATION
LI, J
WIRTZ, RA
MCCONKEY, GA
SATTABONGKOT, J
WATERS, AP
ROGERS, MJ
MCCUTCHAN, TF
[J]. EXPERIMENTAL PARASITOLOGY, 1995, 81 (02) : 182 - 190
[10] ACCURACY OF ROUTINE LABORATORY DIAGNOSIS OF MALARIA IN THE UNITED-KINGDOM
MILNE, LM
KYI, MS
CHIODINI, PL
WARHURST, DC
[J]. JOURNAL OF CLINICAL PATHOLOGY, 1994, 47 (08) : 740 - 742

← 1 2 →