The conserved proteins CHE-12 and DYF-11 cilium function in are required for sensory Caenorhabditis elegans

被引:34
作者
Bacaj, Taulant [1 ]
Lu, Yun [1 ]
Shaham, Shai [1 ]
机构
[1] Rockefeller Univ, Lab Dev Genet, New York, NY 10065 USA
关键词
D O I
10.1534/genetics.107.082453
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Sensory neuron cilia are evolutionarily conserved dendritic appendages that convert environmental stimuli into neuronal activity. Although several cilia components are known, the functions of many remain uncharacterized. Furthermore, the basis of morphological and functional differences between cilia remains largely unexplored. To understand the molecular basis of cilia morphogenesis and function, we studied the Caenorhabditis elegans mutants che-12 and dyf-11. These mutants fail to concentrate lipophilic dyes front their surroundings in sensory neurons and are chemotaxis defective. In che-12 mutants, sensory neuron cilia lack distal segments, while in dyf-11 animals, media] and distal segments are absent. CHE-12 and DYF-11 are conserved ciliary proteins that function cell-autonomously and are continuously required for maintenance of cilium morphology and function. CHE-12, composed primarily of HEAT repeats, may not be part of the intraflagellar transport (IFT) complex and is not required for the localization of some IFT components. DYF-11 undergoes IFT-like movement and may function at an early stage of IFT-B particle assembly. Intriguingly, while DYF-11 is expressed in all C. elegans ciliated neurons, CHE-12 expression is restricted to some amphid sensory neurons, suggesting a specific role in these neurons. Our results provide insight into general and neuron-specific aspects of cilium development and function.
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页码:989 / 1002
页数:14
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