Quantitative PCR method for diagnosis of citrus bacterial canker

被引：35

作者：

Cubero, J

Graham, JH

Gottwald, TR

机构：

[1] Univ Florida, CREC, Lake Alfred, FL 33850 USA

[2] USDA ARS, USHRL, Ft Pierce, FL 34945 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 06期

关键词：

D O I：

10.1128/AEM.67.6.2849-2852.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

For diagnosis of citrus bacterial canker by PCR an internal standard is employed to ensure the quality of the DNA extraction and that proper requisites exist for the amplification reaction. The ratio of PCR products from the internal standard and bacterial target is used to estimate the initial bacterial concentration in citrus tissues with lesions.

引用

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页码：2849 / 2852

页数：4

相关论文

共 17 条

[1]

CIVEROLO E L, 1984, Journal of the Rio Grande Valley Horticultural Society, V37, P127

[2] A simple and efficient PCR method for the detection of Agrobacterium tumefaciens in plant tumours [J].

Cubero, J ;

Martínez, MC ;

Llop, P ;

López, MM .

JOURNAL OF APPLIED MICROBIOLOGY, 1999, 86 (04) :591-602

[3] POPULATION-DYNAMICS OF STRAINS OF XANTHOMONAS-CAMPESTRIS DIFFERING IN AGGRESSIVENESS ON SWINGLE CITRUMELO AND GRAPEFRUIT [J].

EGEL, DS ;

GRAHAM, JH ;

RILEY, TD .

PHYTOPATHOLOGY, 1991, 81 (06) :666-671

[4] VARIATION IN AGGRESSIVENESS OF XANTHOMONAS-CAMPESTRIS PV CITRUMELO ASSOCIATED WITH CITRUS BACTERIAL SPOT IN FLORIDA CITRUS NURSERIES [J].

GRAHAM, JH ;

GOTTWALD, TR .

PHYTOPATHOLOGY, 1990, 80 (02) :190-196

[5] CULTIVAR-SPECIFIC INTERACTIONS FOR STRAINS OF XANTHOMONAS-CAMPESTRIS FROM FLORIDA THAT CAUSE CITRUS CANKER AND CITRUS BACTERIAL SPOT [J].

GRAHAM, JH ;

GOTTWALD, TR ;

FARDELMANN, D .

PLANT DISEASE, 1990, 74 (10) :753-756

[6] SURVIVAL OF XANTHOMONAS-CAMPESTRIS PV CITRI IN CITRUS PLANT DEBRIS AND SOIL IN FLORIDA AND ARGENTINA [J].

GRAHAM, JH ;

MCGUIRE, RG ;

MILLER, JW .

PLANT DISEASE, 1987, 71 (12) :1094-1098

[7] DETECTION OF XANTHOMONAS-CAMPESTRIS PV CITRI BY THE POLYMERASE CHAIN-REACTION METHOD [J].

HARTUNG, JS ;

DANIEL, JF ;

PRUVOST, OP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (04) :1143-1148

[8] Rapid and sensitive colorimetric detection of Xanthomonas axonopodis pv citri by immunocapture and a nested-polymerase chain reaction assay [J].

Hartung, JS ;

Pruvost, OP ;

Villemot, I ;

Alvarez, A .

PHYTOPATHOLOGY, 1996, 86 (01) :95-101

[9] THE POLYMERASE CHAIN-REACTION AND PLANT-DISEASE DIAGNOSIS [J].

HENSON, JM ;

FRENCH, R .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 1993, 31 :81-109

[10] A simple extraction procedure for efficient routine detection of pathogenic bacteria in plant material by polymerase chain reaction [J].

Llop, P ;

Caruso, P ;

Cubero, J ;

Morente, C ;

López, MM .

JOURNAL OF MICROBIOLOGICAL METHODS, 1999, 37 (01) :23-31