Tissue-specific expression of ALA synthase-1 and heme oxygenase-1 and their expression in livers of rats chronically exposed to ethanol

被引：11

作者：

Zheng, Jianyu ^{[1
,2
]}

Tian, Qing ^{[1
]}

Hou, Weihong ^{[1
,2
]}

Watts, John A. ^{[1
,2
]}

Schrum, Laura W. ^{[2
]}

Bonkovsky, Herbert L. ^{[1
,2
,3
]}

机构：

[1] Carolinas Med Ctr, Liver Biliary Pancreat Ctr, Charlotte, NC 28203 USA

[2] Univ N Carolina, Dept Biol, Charlotte, NC 28223 USA

[3] Univ Connecticut, Ctr Hlth, Dept Med, Farmington, CT 06030 USA

来源：

FEBS LETTERS | 2008年 / 582卷 / 13期

关键词：

5-aminolevulinic acid synthase-1 (ALAS1); heme oxygenase-1 (HO-1); ethanol; gene expression; porphyria; rat liver;

D O I：

10.1016/j.febslet.2008.04.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-Aminolevulinic acid synthase-1 (ALAS1) and heme oxygenase-1 (HO-1) are the rate-controlling enzymes for heme biosynthesis and degradation, respectively. Expression of these two genes showed tissue-specific expression pattern at both mRNA and protein levels in selected non-treated rat tissues. In the livers of rats receiving oral ethanol for 10 weeks, ALAS1 mRNA levels were increased by 65%, and the precursor and mature ALAS1 protein levels were increased by 1.8- and 2.3-fold, respectively, while no changes were observed in HO-1 mRNA and protein levels, compared with pair-fed controls. These results provide novel insights into the effects of chronic ethanol consumption on hepatic heme biosynthesis and porphyrias. (c) 2008 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：1829 / 1834

页数：6

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