Endogenous pacemaker activity of rat tumour somatotrophs

1. Cells derived from a rat pituitary tumour (GC cell line) that continuously release growth hormone behave as endogenous pacemakers. In simultaneous patch clamp recordings and cytosolic Ca2+ concentration ([Ca2+](1)) imaging, they displayed rhythmic action potentials (44.7 +/- 2.7 mV, 178 +/- 40ms, 0.30 +/- 0.04 Hz) and concomitant [Ca2+], transients (374 +/- 57 nM, 1.0 +/- 0.2 s, 0.27 +/- 0.03 Hz). 2. Action potentials and [Ca2+](1) transients were reversibly blocked by removal of external Ca2+, addition of nifedipine (1 mu M) or Ni2+ (40 mu M), but were insensitive to TTX (1 mu M). An L-type Ca2+ current activated at -33.6 +/- 0.4 mV (holding potential (V-h), -40 mV), peaked at -1.8 +/- 1.3 mV, was reduced by nifedipine and enhanced by S(+)-SDZ 202 791. A T/R-type Ca2+ current activated at -41.7 +/- 2.7 mV (V-h, -80 or -60 mV), peaked at -9.2 +/- 3.0 mV, was reduced by low concentrations of Ni2+ (40 mu M) or Cd2+ (10 mu M) and was toxin resistant. Parallel experiments revealed the expression of the class E calcium channel alpha 1-subunit mRNA. 3. The K+ channel blockers TEA (25 mM) and charybdotoxin (10-100 nM) enhanced spike amplitude and/or duration. Apamin (100 nM) also strongly reduced the after-spike hyperpolarization. The outward K+ tail current evoked by a depolarizing step that mimicked an action potential reversed at -69.8 +/- 0.3 mV, presented two components, lasted 2-3 s and was totally blocked by Cd2+ (400 mu M). 4. The slow pacemaker depolarization (3.5 +/- 0.4 s) that separated consecutive spikes corresponded to a 2- to 3-fold increase in membrane resistance, was strongly Naf sensitive but TTX insensitive. 5. Computer simulations showed that pacemaker activity can be reproduced by a minimum of six currents: an L-type Ca2+ current underlies the rising phase of action potentials that are repolarized by a delayed rectifier and Ca2+-activated K+ currents. In between spikes, the decay of Ca2+-activated K+ currents and a persistent inward cationic current depolarize the membrane, activate the T/R-type Ca2+ current and initiate a new cycle.