Preparation of polyvinyl alcohol-polyacrylamide composite polymer membrane by γ-irradiation for entrapment of urease

被引：21

作者：

Jha, SK ^{[1
]}

D'Souza, SF ^{[1
]}

机构：

[1] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Bombay 400085, Maharashtra, India

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2005年 / 62卷 / 03期

关键词：

polyvinyl alcohol; polyacrylamide; gamma-irradiation; ureasc; polymer membrane; entrapment;

D O I：

10.1016/j.jbbm.2004.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Composite polymer membrane of polyvinyl alcohol (PVA) and acrylamide was prepared on cheesecloth support by gamma-irradiation induced free radical polymerization. The enzyme urease was entrapped in the membrane during polymerization and was cross-linked within the matrix using glutaraldehyde. The membranes could be reused a number of times without significant loss of urease activity. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：215 / 218

页数：4

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