Constructs and methods for hairpin RNA-mediated gene silencing in plants

被引：73

作者：

Helliwell, CA

Waterhouse, PM

机构：

来源：

RNA INTERFERENCE | 2005年 / 392卷

关键词：

D O I：

10.1016/S0076-6879(04)92002-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Double-stranded RNA (dsRNA) induces an endogenous sequence-specific RNA degradation mechanism in most eukaryotic cells. The mechanism can be harnessed to silence genes in plants by expressing self-complementary single-stranded (hairpin) RNA in which the duplexed region has the same sequence as part of the target gene's mRNA. We describe a number of plasmid vectors for generating hairpin RNAs, including those designed for high-throughput cloning, and provide protocols for their use.

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页码：24 / 35

页数：12

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