Production of a recombinant xylanase in plants and its potential for pulp biobleaching applications

被引：34

作者：

Bae, Hyeun-Jong ^{[1
]}

Kim, Hyun Joo ^{[1
]}

Kim, Yoon Soo ^{[1
]}

机构：

[1] Chonnam Natl Univ, Program BK21, Dept Forest Prod & Technol, Kwangju 500757, South Korea

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 09期

关键词：

biobleaching; plant expression; transgenic plant; xylanase;

D O I：

10.1016/j.biortech.2007.07.064

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The purpose of this study was to produce recombinant xylanase in transgenic plants and to test its potential application for pulp bleaching. The xynII xylanase gene from Trichoderma reesei was inserted into the Arabidopsis genome. Many transgenic plants produced biologically active XYNII and accumulated in leaves at level of 1.4-3.2% of total soluble proteins. The bleaching ability of XYNII on Kraft pulp was demonstrated by a reduction in the kappa number and the residual lignin contents. The bleaching efficiency of transgenic plant produced XYNII was similar to commercial xylanase on unbleached Kraft pulp. The effect of xylanase treatment on Kraft pulp was also investigated by SEM. Clear physical change on the pulp fiber surface was observed and was related to the amount xylan removed and microfibrils were visible on the fiber surface. This report demonstrates the potential application of plant produced recombinant xylanase for pulp and paper bleaching. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3513 / 3519

页数：7

共 25 条

[1]

AUFFRAY C, 1980, EUR J BIOCHEM, V107, P303

[2]

BAE H, 2006, J EXP BOT, V57, P161

[3] Enhanced production of a thermostable xylanase from Streptomyces sp QG-11-3 and its application in biobleaching of eucalyptus kraft pulp [J].

Beg, QK ;

Bhushan, B ;

Kapoor, M ;

Hoondal, GS .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (07) :459-466

[4]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[5] Green colour protection of makino bamboo (Phyllostachys makinoi) treated with ammoniacal copper quaternary and copper azole preservatives [J].

Chung, MJ ;

Wu, JH ;

Chang, ST .

POLYMER DEGRADATION AND STABILITY, 2005, 90 (01) :167-172

[6] A comparison of enzyme-aided bleaching of softwood paper pulp using combinations of xylanase, mannanase and α-galactosidase [J].

Clarke, JH ;

Davidson, K ;

Rixon, JE ;

Halstead, JR ;

Fransen, MP ;

Gilbert, HJ ;

Hazlewood, GP .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2000, 53 (06) :661-667

[7] Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].

Clough, SJ ;

Bent, AF .

PLANT JOURNAL, 1998, 16 (06) :735-743

[8]

De Vries S., 1988, PLANT MOL BIOL MANUA, P1

[9] Protein modifications in the plant secretory pathway: current status and practical implications in molecular pharming [J].

Faye, L ;

Boulaflous, A ;

Benchabane, M ;

Gomord, W ;

Michaud, D .

VACCINE, 2005, 23 (15) :1770-1778

[10] Enzymatic removal of hemicellulose from dissolving pulps [J].

Gubitz, GM ;

Lischnig, T ;

Stebbing, D ;

Saddler, JN .

BIOTECHNOLOGY LETTERS, 1997, 19 (05) :491-495

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