Apigenin inhibits the production of NO and PGE2 in microglia and inhibits neuronal cell death in a middle cerebral artery occlusion-induced focal ischemia mice model

被引：158

作者：

Ha, Sang Keun ^{[1
]}

Lee, Pyeongjae ^{[2
]}

Park, Jeong A. ^{[1
]}

Oh, Hye Rim ^{[3
]}

Lee, Sang Yeon ^{[1
]}

Park, Ji-Ho ^{[1
]}

Lee, Eunjoo H. ^{[1
]}

Ryu, Jong Hoon ^{[3
]}

Lee, Kang Ro ^{[4
]}

Kim, Sun Yeou ^{[1
]}

机构：

[1] Kyung Hee Univ, Grad Sch EW Med Sci, Kihung Ku, Yongin 449701, Kyungki Do, South Korea

[2] Semyung Univ, Dept Nat Med Resource, Jecheon City 390711, Chungbuk, South Korea

[3] Kyung Hee Univ, Dept Oriental Pharmaceut Sci, Seoul, South Korea

[4] Sungkyunkwan Univ, Coll Pharm, Suwon 440746, South Korea

来源：

NEUROCHEMISTRY INTERNATIONAL | 2008年 / 52卷 / 4-5期

关键词：

flavonoid; nitric oxide; apigenin; inflammation; prostaglandin E2; mitogen-activated protein kinase; middle cerebral artery occlusion;

D O I：

10.1016/j.neuint.2007.10.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Flavonoids have been intensively studied on their pharmacological activities such as anti-cancer, anti-oxidant and anti-inflammation. However, little is known about their neuroprotective effects. Recent studies suggest that inflammation mediated by microglia may play a role in neurodegenerative diseases. In this study, we evaluated the anti-inflammatory effect of various flavonoid compounds by using BV-2, a murine microglia cell line. Of the compounds that were evaluated, apigenin inhibited the production of nitric oxide and prostaglandin E-2 by suppressing the expression of inducible nitric oxide synthase and cyclooxygenase-2 protein, respectively. Moreover, apigenin suppressed p38 mitogen-activated protein kinase (MAPK), c-Jun N-terminal kinase (JNK) phosphorylation without affecting the activity of extracellular signal-regulated kinase (ERK). Apigenin was also found to protect neuronal cells from injury in middle cerebral artery occlusion. (C) 2007 Published by Elsevier ltd.

引用

页码：878 / 886

页数：9

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