Reduced hydrogen peroxide production in neutrophils from patients with diabetes

被引:30
作者
Inoue, S [1 ]
Lan, Y [1 ]
Muran, J [1 ]
Tsuji, M [1 ]
机构
[1] HOKKAIDO CENT HOSP SOCIAL HLTH INCIDENCE,DEPT INTERNAL MED,SAPPORO,HOKKAIDO 062,JAPAN
关键词
hydrogen peroxide production; neutrophils; diabetes mellitus; phorbol myristate acetate; colony-stimulating factor;
D O I
10.1016/0168-8227(96)01287-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We evaluated the effects of granulocyte colony-stimulating factors (G-CSF), granulocyte-macrophage colony-stimulating factors (GM-CSF) and phorbol myristate acetate (PMA) on H2O2 production in purified neutrophils from patients with diabetes mellitus (DM), using flow cytometry. Twenty-two age-matched male subjects were selected: 11 were normal volunteers and the remainder had DM. No significant differences in intracellular H2O2 production per 60 min was observed in the 'resting' neutrophils from DM patients (37.2 +/- 20.4 A.U.) compared with those from normal volunteers (24.9 +/- 8.4 A.U.). The PMA-stimulated neutrophils from normal volunteers generated approximately 4-fold increases in H2O2 per 60 min compared with those from DM patients. Under similar culture conditions, G-CSF caused 1.6-fold increases of H2O2 in neutrophils from normal volunteers compared with those of DM patients. Increases after GM-CSF stimulation were 2-fold higher in volunteer neutrophils compared with those from DM patients. The levels of G-CSF- or GM-CSF-stimulated H2O2 production in neutrophils from DM patients were low and were little different from non-stimulated resting cells. These data showed that H2O2 production in neutrophils induced by PMA is impaired in patients with DM, and neither G-CSF nor GM-CSF enhances its production.
引用
收藏
页码:119 / 127
页数:9
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