Effects of apolipoprotein B-100 on the metabolism of a lipid microemulsion model in rats

被引:28
作者
Hirata, RDC
Hirata, MH
Mesquita, CH
Cesar, TB
Maranhao, RC
机构
[1] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin & Toxicol Anal, BR-05500890 Sao Paulo, SP, Brazil
[2] Univ Sao Paulo, Med Sch Hosp, Inst Heart, Sao Paulo, Brazil
[3] UNESP, Fac Pharmaceut Sci, Sao Paulo, Brazil
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 1999年 / 1437卷 / 01期
基金
巴西圣保罗研究基金会;
关键词
apolipoprotein B-100; low density lipoprotein; metabolism; microemulsion; plasma kinetics; estradiol;
D O I
10.1016/S1388-1981(98)00004-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In previous studies, it was shown that lipid microemulsions resembling LDL (LDE) but not containing protein, acquire apolipoprotein E when injected into the bloodstream and bind to LDL receptors (LDLR) using this protein as ligand. Aiming to evaluate the effects of apolipoprotein (apo) B-100 on the catabolism of these microemulsions, LDE with incorporated apo B-100 (LDE-apoB) and native LDL, all labeled with radioactive lipids were studied after intraarterial injection into Wistar rats. Plasma decay curves of the labels were determined in samples collected over 10 h and tissue uptake was assayed from organs excised from the animals sacrificed 24 h after injection. LDE-apo B had a fractional clearance rate (FCR) similar to native LDL (0.40 and 0.33, respectively) but both had FCR pronouncedly smaller than LDE (0.56, P<0.01). Liver was the main uptake site for LDE, LDE-apoB, and native LDL, but LDE-apoB and native LDL had lower hepatic uptake rates than LDE. Pre-treatment of the rats with 17 alpha-ethinylestradiol, known to upregulate LDLR, accelerated the removal from plasma of both LDE and LDE-apoB, but the effect was greater upon LDE than LDE-apoB. These differences in metabolic behavior documented in vivo can be interpreted by the lower affinity of LDLR for apo B-100 than for apo E, demonstrated in in vitro studies. Therefore, our study shows in vivo that, in comparison with apo E, apo B is a less efficient ligand to remove lipid particles such as microemulsions or lipoproteins from the intravascular compartment. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:53 / 62
页数:10
相关论文
共 51 条
[1]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[2]  
Bertolotti M, 1996, J LIPID RES, V37, P1812
[3]   ROLE OF THE LIVER IN LOW-DENSITY-LIPOPROTEIN CATABOLISM IN THE RAT [J].
BHATTACHARYA, S ;
BALASUBRAMANIAM, S ;
SIMONS, LA .
BIOCHEMICAL JOURNAL, 1984, 220 (01) :333-336
[4]   REGULATION OF LOW-DENSITY-LIPOPROTEIN METABOLISM IN THE RAT [J].
BHATTACHARYA, S ;
BALASUBRAMANIAM, S ;
SIMONS, LA .
BIOCHEMICAL JOURNAL, 1986, 234 (02) :493-496
[5]   A RECEPTOR-MEDIATED PATHWAY FOR CHOLESTEROL HOMEOSTASIS [J].
BROWN, MS ;
GOLDSTEIN, JL .
SCIENCE, 1986, 232 (4746) :34-47
[6]  
CASTER WO, 1956, P SOC EXP BIOL MED, V91, P122, DOI 10.3181/00379727-91-22186
[7]  
CHAO Y, 1979, J BIOL CHEM, V254, P1360
[8]  
CHAPMAN MJ, 1986, METHOD ENZYMOL, V128, P70
[9]   Control of apolipoprotein B mRNA editing: Implications of mRNA dynamics at various maturation stages [J].
Chen, L ;
Chan, L .
JOURNAL OF THEORETICAL BIOLOGY, 1996, 183 (04) :391-407
[10]   APOLIPOPROTEIN B-48 IS THE PRODUCT OF A MESSENGER-RNA WITH AN ORGAN-SPECIFIC IN-FRAME STOP CODON [J].
CHEN, SH ;
HABIB, G ;
YANG, CY ;
GU, ZW ;
LEE, BR ;
WENG, SA ;
SILBERMAN, SR ;
CAI, SJ ;
DESLYPERE, JP ;
ROSSENEU, M ;
GOTTO, AM ;
LI, WH ;
CHAN, L .
SCIENCE, 1987, 238 (4825) :363-366