Diversity surveys and evolutionary relationships of aoxB genes in aerobic arsenite-oxidizing bacteria

被引：128

作者：

Quemeneur, Marianne ^{[1
]}

Heinrich-Salmeron, Audrey ^{[2
,3
]}

Muller, Daniel ^{[2
,3
,4
,5
]}

Livremont, Didier ^{[2
,3
]}

Jauzein, Michel ^{[6
,7
]}

Bertin, Philippe N. ^{[2
,3
]}

Garrido, Francis ^{[1
]}

Joulian, Catherine ^{[1
]}

机构：

[1] Bur Rech Geol & Minieres, Ecotechnol Unit, Proc & Environm Div, F-45060 Orleans 02, France

[2] CNRS, UMR 7156, F-67000 Strasbourg, France

[3] Univ Strasbourg, F-67000 Strasbourg, France

[4] Ecol Microbienne, CNRS, UMR 5557, USC INRA 1193, F-69622 Villeurbanne, France

[5] Univ Lyon, F-69622 Villeurbanne, France

[6] CNRS, UMR 7137, LIMOS, F-54506 Vandoeuvre Les Nancy, France

[7] Univ Nancy, F-54506 Vandoeuvre Les Nancy, France

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 14期

关键词：

D O I：

10.1128/AEM.02851-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new primer set was designed to specifically amplify ca. 1,100 bp of aoxB genes encoding the As(III) oxidase catalytic subunit from taxonomically diverse aerobic As(III)-oxidizing bacteria. Comparative analysis of AoxB protein sequences showed variable conservation levels and highlighted the conservation of essential amino acids and structural motifs. AoxB phylogeny of pure strains showed well-discriminated taxonomic groups and was similar to 16S rRNA phylogeny. Alphaproteobacteria-, Betaproteobacteria-, and Gammaproteobacteria-related sequences were retrieved from environmental surveys, demonstrating their prevalence in mesophilic As-contaminated soils. Our study underlines the usefulness of the aoxB gene as a functional marker of aerobic As(III) oxidizers.

引用

页码：4567 / 4573

页数：7

共 44 条

[1]

ANDERSON GL, 1992, J BIOL CHEM, V267, P23674

[2]

[Anonymous], 1989, Cladistics, DOI DOI 10.1111/J.1096-0031.1989.TB00562.X

[3] An arsenic(III)-oxidizing bacterial population: selection, characterization, and performance in reactors [J].

Battaglia-Brunet, F ;

Dictor, MC ;

Garrido, F ;

Crouzet, C ;

Morin, D ;

Dekeyser, K ;

Clarens, M ;

Baranger, P .

JOURNAL OF APPLIED MICROBIOLOGY, 2002, 93 (04) :656-667

[4] A simple biogeochemical process removing arsenic from a mine drainage water [J].

Battaglia-Brunet, Fabienne ;

Itard, Yann ;

Garrido, Francis ;

Delorme, Fabian ;

Crouzet, Catherine ;

Greffie, Catherine ;

Joulian, Catherine .

GEOMICROBIOLOGY JOURNAL, 2006, 23 (3-4) :201-211

[5] Oxidation of arsenite by Thiomonas strains and characterization of Thiomonas arsenivorans sp nov. [J].

Battaglia-Brunet, Fabienne ;

Joulian, Catherine ;

Garrido, Francis ;

Dictor, Marie-Christine ;

Morin, Dominique ;

Coupland, Kris ;

Johnson, D. Barrie ;

Hallberg, Kevin B. ;

Baranger, Philippe .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2006, 89 (01) :99-108

[6] Nitrite reductase genes (nirK and nirS) as functional markers to investigate diversity of denitrifying bacteria in Pacific northwest marine sediment communities [J].

Braker, G ;

Zhou, JZ ;

Wu, LY ;

Devol, AH ;

Tiedje, JM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (05) :2096-2104

[7] Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies [J].

Case, Rebecca J. ;

Boucher, Yan ;

Dahllof, Ingela ;

Holmstrom, Carola ;

Doolittle, W. Ford ;

Kjelleberg, Staffan .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2007, 73 (01) :278-288

[8]

Coupland K, 2004, BIOHYDROMETALLURGY S, P639

[9] Arsenite-oxidizing Hydrogenobaculum strain isolated from an acid-sulfate-chloride geothermal spring in Yellowstone National Park [J].

Donahoe-Christiansen, J ;

D'Imperio, S ;

Jackson, CR ;

Inskeep, WP ;

McDermott, TR .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (03) :1865-1868

[10] Crystal structure of the 100 kDa arsenite oxidase from Alcaligenes faecalis in two crystal forms at 1.64 Å and 2.03 Å [J].

Ellis, PJ ;

Conrads, T ;

Hille, R ;

Kuhn, P .

STRUCTURE, 2001, 9 (02) :125-132

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