The chaperone/usher pathways of Pseudomonas aeruginosa:: Identification of fimbrial gene clusters (cup) and their involvement in biofilm formation

被引:236
作者
Vallet, I
Olson, JW
Lory, S
Lazdunski, A
Filloux, A
机构
[1] CNRS, Inst Biol Struct & Microbiol, UPR 9027, Lab Ingn Syst Macromol, F-13402 Marseille 20, France
[2] Harvard Univ, Sch Med, Dept Microbiol & Mol Genet, Boston, MA 02115 USA
关键词
D O I
10.1073/pnas.111551898
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pseudomonas aeroginosa, an important opportunistic human pathogen, persists in certain tissues in the form of specialized bacterial communities, referred to as biofilm, The biofilm is formed through series of interactions between cells and adherence to surfaces, resulting in an organized structure, By screening a library of Tn5 insertions in a nonpiliated P. aeruginosa strain, we identified genes involved in early stages of biofilm formation. One class of mutations identified in this study mapped in a cluster of genes specifying the components of a chaperone/usher pathway that is involved in assembly of fimbrial subunits in other microorganisms, These genes, not previously described in P. aeruginosa, were named cupA1-A5. Additional chaperone/usher systems (CupB and CupC) have been also identified in the genome of P. aeruginosa PAO1; however, they do not appear to play a role in adhesion under the conditions where the CupA system is expressed and functions in surface adherence. The identification of these putative adhesins on the cell surface of P, aeruginosa suggests that this organism possess a wide range of factors that function in biofilm formation. These structures appear to be differentially regulated and may function at distinct stages of biofilm formation, or in specific environments colonized by this organism.
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页码:6911 / 6916
页数:6
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