A rapid method for determining the tuberculocidal activity of liquid chemical germicides

被引：6

作者：

Erickson, BD ^{[1
]}

Campbell, WL ^{[1
]}

Cerniglia, CE ^{[1
]}

机构：

[1] US FDA, Natl Ctr Toxicol Res, Div Microbiol, Jefferson, AR 72079 USA

来源：

CURRENT MICROBIOLOGY | 2001年 / 43卷 / 02期

关键词：

D O I：

10.1007/s002840010264

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A rapid, quantitative method has been developed for determining the tuberculocidal activity of liquid chemical germicides. In this method, a test strain of Mycobacterium bovis that carries the firefly luciferase gene is exposed to a,germicide, and the surviving bacteria are detected by bioluminescence. The tuberculocidal activities of five commercially available glutaraldehyde-based disinfectants were tested, and all reduced the number of surviving mycobacteria by greater than five orders of magnitude. In contrast, a phenol-based disinfectant with tuberculocidal claims gave less than one order of magnitude reduction of the test organism. With this method for determining tuberculocidal activity, results can be obtained in less than one day, compared with weeks or months for the standard tuberculocidal assays.

引用

页码：79 / 82

页数：4

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