Intracellular detection assays for high-throughput screening

被引:53
作者
González, JE [1 ]
Negulescu, PA [1 ]
机构
[1] Aurora Biosci Corp, San Diego, CA 92121 USA
关键词
D O I
10.1016/S0958-1669(98)80141-9
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-engineered biosensors for intracellular biochemistry, specifically localized targets, and protein-protein interactions.
引用
收藏
页码:624 / 631
页数:8
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