C-13 nuclear magnetic resonance analysis of glucose and citrate end products in an ldhL-ldhD double-knockout strain of Lactobacillus plantarum

被引：65

作者：

Ferain, T

Schanck, AN

Delcour, J

机构：

[1] UNIV CATHOLIQUE LOUVAIN, MOL GENET LAB, UNITE GENET, B-1348 LOUVAIN, BELGIUM

[2] UNIV CATHOLIQUE LOUVAIN, CHIM PHYS & CRISTALLOG LAB, B-1348 LOUVAIN, BELGIUM

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 24期

关键词：

D O I：

10.1128/jb.178.24.7311-7315.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have examined the metabolic consequences of knocking out the two ldh genes in Lactobacillus plantarum using C-13 nuclear magnetic resonance. Unlike its wild-type isogenic progenitor, which produced lactate as the major metabolite under all conditions tested, ldh null strain TF103 mainly produced acetoin, A variety of secondary end products were also found, including organic acids (acetate, succinate, pyruvate, and lactate), ethanol, 2,3-butanediol, and mannitol.

引用

页码：7311 / 7315

页数：5

共 42 条

[1] NAD+-DEPENDENT D-2-HYDROXYISOCAPROATE DEHYDROGENASE OF LACTOBACILLUS-DELBRUECKII SUBSP BULGARICUS - GENE CLONING AND ENZYME CHARACTERIZATION
BERNARD, N
JOHNSEN, K
FERAIN, T
GARMYN, D
HOLS, P
HOLBROOK, JJ
DELCOUR, J
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 224 (02): : 439 - 446
[2] CASPRITZ G, 1983, J BIOL CHEM, V258, P4907
[3] MEDIUM FOR SCREENING LEUCONOSTOC-OENOS STRAINS DEFECTIVE IN MALOLACTIC FERMENTATION
CAVIN, JF
PREVOST, H
LIN, J
SCHMITT, P
DIVIES, C
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (03) : 751 - 753
[4] METABOLISM OF MANNITOL + INDUCTION OF MANNITOL 1-PHOSPHATE DEHYDROGENASE IN LACTOBACILLUS PLANTARUM
CHAKRAVORTY, M
[J]. JOURNAL OF BACTERIOLOGY, 1964, 87 (05) : 1246 - &
[5] MALATE-DEHYDROGENASE FROM CHLOROBIUM-VIBRIOFORME, CHLOROBIUM-TEPIDUM, AND HELIOBACTERIUM-GESTII - PURIFICATION, CHARACTERIZATION, AND INVESTIGATION OF DINUCLEOTIDE BINDING BY DEHYDROGENASES BY USE OF EMPIRICAL-METHODS OF PROTEIN-SEQUENCE ANALYSIS
CHARNOCK, C
REFSETH, UH
SIREVAG, R
[J]. JOURNAL OF BACTERIOLOGY, 1992, 174 (04) : 1307 - 1313
[6] AEROBIC AND ANAEROBIC GLUCOSE-METABOLISM OF PHYTOMONAS SP ISOLATED FROM EUPHORBIA-CHARACIAS
CHAUMONT, F
SCHANCK, AN
BLUM, JJ
OPPERDOES, FR
[J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 67 (02) : 321 - 331
[7] CHEN K-H, 1986, Food Microbiology (London), V3, P83, DOI 10.1016/S0740-0020(86)80030-2
[8] CONDON S, 1987, FEMS MICROBIOL LETT, V46, P269, DOI 10.1111/j.1574-6968.1987.tb02465.x
[9] ELGENDY SM, 1983, J FOOD PROTECT, V46, P503, DOI 10.4315/0362-028X-46.6.503
[10] Knockout of the two ldh genes has a major impact on peptidoglycan precursor synthesis in Lactobacillus plantarum
Ferain, T
Hobbs, JN
Richardson, J
Bernard, N
Garmyn, D
Hols, P
Allen, NE
Delcour, J
[J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (18) : 5431 - 5437

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