Interactions of acid phosphatase with clays, organic molecules and organo-mineral complexes

被引:60
作者
Rao, MA [1 ]
Gianfreda, L [1 ]
Palmiero, F [1 ]
Violante, A [1 ]
机构
[1] UNIV NAPLES FEDERICO II, DIPARTIMENTO SCI CHIM AGR, I-80055 PORTICI, ITALY
关键词
D O I
10.1097/00010694-199611000-00004
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Synthetic active enzymatic complexes have been formed by the interaction of acid phosphatase with clays (montmorillonite and chlorites), organic molecules, and organo-mineral associations, The inhibition of each component on the activity of the free enzyme and the residual activity of the enzymatic complexes have both been determined, Clays and tannic acid displayed various inhibitory effects on acid phosphatase activity, Montmorillonite and aluminum hydrous oxide showed the highest (70%) and the lowest (<5%) inhibitory influence, respectively, The covering of montmorillonite surfaces with OH-Al species increased not only the amount but also the residual activity of adsorbed acid phosphatase molecules. When 18 meg of Al g(-1) clay were adsorbed on montmorillonite surfaces, the residual activity of adsorbed enzyme increased up to 54% of that added initially, The activity retained by tannate-acid phosphatase complexes depended on incubation time and tannic acid/enzyme ratios, This suggests that a complexation between tannic acid and enzyme molecules, rather than a polymerization process of tannic acid involving acid phosphatase, took place, The presence of OH-Al species during the interaction between the two components favored the entrapment of more enzymatic molecules at higher activity levels.
引用
收藏
页码:751 / 760
页数:10
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