A mechanism for the regulation of pre-mRNA 3′ processing by human cleavage factor Im

被引：165

作者：

Brown, KM ^{[1
]}

Gilmartin, GM ^{[1
]}

机构：

[1] Univ Vermont, Dept Microbiol & Mol Genet, Burlington, VT 05405 USA

来源：

MOLECULAR CELL | 2003年 / 12卷 / 06期

关键词：

D O I：

10.1016/S1097-2765(03)00453-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human cleavage factor I-m (CFIm) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFIm can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-dependent manner. Elevated levels of CFIm suppressed cleavage at the primary poly(A) site of the pre-mRNA encoding the 68 kDa subunit of CFIm. CFIm-mediated suppression of poly(A) site cleavage was dependent upon the presence of three copies of an RNA element initially identified by CFIm-SELEX. These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor.

引用

页码：1467 / 1476

页数：10

共 36 条

[1] Patterns of variant polyadenylation signal usage in human genes [J].

Beaudoing, E ;

Freier, S ;

Wyatt, JR ;

Claverie, JM ;

Gautheret, D .

GENOME RESEARCH, 2000, 10 (07) :1001-1010

[2] Reduction of target gene expression by a modified U1 snRNA [J].

Beckley, SA ;

Liu, P ;

Stover, ML ;

Gunderson, SI ;

Lichtler, AC ;

Rowe, DW .

MOLECULAR AND CELLULAR BIOLOGY, 2001, 21 (08) :2815-2825

[3] The mRNA assembly line: transcription and processing machines in the same factory [J].

Bentley, D .

CURRENT OPINION IN CELL BIOLOGY, 2002, 14 (03) :336-342

[4] RNA ligands selected by cleavage stimulation factor contain distinct sequence motifs that function as downstream elements in 3'-end processing of pre-mRNA [J].

Beyer, K ;

Dandekar, T ;

Keller, W .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (42) :26769-26779

[5] Strange bedfellows: polyadenylation factors at the promoter [J].

Calvo, O ;

Manley, JL .

GENES & DEVELOPMENT, 2003, 17 (11) :1321-1327

[6] Transcription factor TFIID recruits factor CPSF for formation of 3' end of mRNA [J].

Dantonel, JC ;

Murthy, KGK ;

Manley, JL ;

Tora, L .

NATURE, 1997, 389 (6649) :399-402

[7] A history of poly A sequences: From formation to factors to function [J].

Edmonds, M .

PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 71, 2002, 71 :285-389

[8] Alternative poly(A) site selection in complex transcription units: Means to an end? [J].

EdwaldsGilbert, G ;

Veraldi, KL ;

Milcarek, C .

NUCLEIC ACIDS RESEARCH, 1997, 25 (13) :2547-2561

[9] Inhibiting expression of specific genes in mammalian cells with 5′ end-mutated U1 small nuclear RNAs targeted to terminal exons of pre-mRNA [J].

Fortes, P ;

Cuevas, Y ;

Guan, F ;

Liu, P ;

Pentlicky, S ;

Jung, SP ;

Martínez-Chantar, ML ;

Prieto, J ;

Rowe, D ;

Gunderson, SI .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (14) :8264-8269

[10] An mRNA surveillance mechanism that eliminates transcripts lacking termination codons [J].

Frischmeyer, PA ;

van Hoof, A ;

O'Donnell, K ;

Guerrerio, AL ;

Parker, R ;

Dietz, HC .

SCIENCE, 2002, 295 (5563) :2258-2261

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