Microtubule-assisted mechanism for functional metabolic macromolecular complex formation

被引：77

作者：

An, Songon ^{[1
]}

Deng, Yijun ^{[1
]}

Tomsho, John W. ^{[1
]}

Kyoung, Minjoung ^{[2
]}

Benkovic, Stephen J. ^{[1
]}

机构：

[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA

[2] Stanford Univ, Dept Mol & Cellular Physiol, Stanford, CA 94305 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2010年 / 107卷 / 29期

基金：

美国国家卫生研究院;

关键词：

metabolism; protein complex; purine biosynthesis; GLYCOLYTIC-ENZYMES; PURINE; CELLS;

D O I：

10.1073/pnas.1008451107

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Evidence has been presented for a metabolic multienzyme complex, the purinosome, that participates in de novo purine biosynthesis to form clusters in the cytoplasm of living cells under purine-depleted conditions. Here we identified, using fluorescent live cell imaging, that a microtubule network appears to physically control the spatial distribution of purinosomes in the cytoplasm. Application of a cell-based assay measuring the rate of de novo purine biosynthesis confirmed that the metabolic activity of purinosomes was significantly suppressed in the absence of microtubules. Collectively, we propose a microtubule-assisted mechanism for functional purinosome formation in HeLa cells.

引用

页码：12872 / 12876

页数：5

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