Tumor necrosis factor-α exerts pro-myogenic action in C2C12 myoblasts dvia sphingosine kinase/S1P2 signaling

被引：40

作者：

Donati, Chiara

Nincheri, Paola

Cencetti, Francesca

Rapizzi, Elena

Farnararo, Marta

Bruni, Paola

机构：

[1] Univ Studi Florence, Dipartimento Sci Biochim, I-50134 Florence, Italy

[2] Univ Studi Florence, Inst Interuniv Miologia, I-50134 Florence, Italy

来源：

FEBS LETTERS | 2007年 / 581卷 / 23期

关键词：

tumor necrosis factor-alpha; sphingosine kinase; skeletal muscle differentiation; S1P(2) receptor;

D O I：

10.1016/j.febslet.2007.08.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, we report that low doses of tumor necrosis factor-alpha (TNF alpha) promote myogenesis in C2C12 myoblasts. Moreover, the cytokine increased sphingosine kinase (SphK) activity and induced SphK1 translocation to membranes. The inhibition of SphK functionality by various approaches abrogated the pro-myogenic effect of TNFa. Moreover, silencing of S1P(2) impaired the positive action of TIN Fa on myogenesis. These results represent the first evidence that SphK/S1P(2) axis is required for the regulation of myogenesis by TNFa. In view of the physiological role of TNF alpha in muscle regeneration, the present finding reinforces the notion that SphK/SIP2 signaling is critically implicated in myogenesis. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

收藏

页码：4384 / 4388

页数：5

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