P-glycoprotein-associated chloride currents revealed by specific block by an anti-P-glycoprotein antibody

被引:27
作者
Han, ES [1 ]
Vanoye, CG [1 ]
Altenberg, GA [1 ]
Reuss, L [1 ]
机构
[1] UNIV TEXAS, MED BRANCH, DEPT PHYSIOL & BIOPHYS, GALVESTON, TX 77555 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 1996年 / 270卷 / 05期
关键词
multidrug resistance; chloride channels; cell volume regulation; rhodamine; 123; MCF-7; C219;
D O I
10.1152/ajpcell.1996.270.5.C1370
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The relationships between P-glycoprotein (PGP) expression and plasma membrane ion currents activated by cell swelling were studied in several cell lines by use of the whole cell configuration of the patch-clamp technique. Swelling-activated Cl- currents (I-Cl(S)) had similar characteristics independently of whether PGP was expressed. Addition of the anti-PGP monoclonal antibody C219 or its Fab fragment to the pipette solution prevented I-Cl(S) in cells expressing functional PGP (assessed by immunoblots, immunofluorescence, and transport of rhodamine 123) but not in cells lacking PGP expression. A peptide analogue of the C219 epitope abolished the effect of C219. Other anti-POP antibodies and mouse immunoglobulin G were ineffective. C219 did not alter swelling-activated cation currents. Inasmuch as I-Cl(S) is present in cells that do not express PGP and C219 has no effect on I-Cl(S) in these cells, we conclude that PGP is not required for the I-Cl(S) phenotype. However, when expressed in the plasma membrane, PGP is involved, directly or indirectly, in I-Cl(S) but not in swelling-activated K+ currents.
引用
收藏
页码:C1370 / C1378
页数:9
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