Characterization of cellular uptake and distribution of vitamin E

被引：54

作者：

Saito, Y ^{[1
]}

Yoshida, Y ^{[1
]}

Nishio, K ^{[1
]}

Hayakawa, M ^{[1
]}

Niki, E ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Human Stress Signal Res Ctr, Ikeda, Osaka 5638577, Japan

来源：

VITAMIN E AND HEALTH | 2004年 / 1031卷

关键词：

vitamin E; tocotrienol; cellular uptake; oxidative stress; antioxidant;

D O I：

10.1196/annals.1331.047

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We previously reported that tocotrienols acted as more potent inhibitors against selenium deficiency-induced cell death than the corresponding tocopherol isoforms (J. BioL Chem. 2003;278:39428-39434). In the present study, we first compared the differences in the cellular uptake between alpha-tocopherol (alpha-Toc) and alpha-tocotrienol (alpha-Toc-3). The initial rate of cellular uptake of alpha-Toc-3 was 70-fold higher than that of alpha-Toc. Subcellular fractionation analysis of alpha-Toc-3 and alpha-Toc-fortified cells showed similar cellular distribution of these antioxidants, which was directly proportional to the lipid distribution. The cells containing similar amounts of alpha-Toc-3 and alpha-Toc showed similar resistance against the oxidative stress caused by peroxides. These results suggest that the apparent higher cytoprotective effect of alpha-Toc-3 than alpha-Toc is primarily ascribed to its higher cellular uptake.

引用

页码：368 / 375

页数：8

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