Evidence of peroxynitrite involvement in the carrageenan-induced rat paw edema

被引：208

作者：

Salvemini, D ^{[1
]}

Wang, ZQ ^{[1
]}

Bourdon, DM ^{[1
]}

Stern, MK ^{[1
]}

Currie, MG ^{[1
]}

Manning, PT ^{[1
]}

机构：

[1] MONSANTO CORP RES, ST LOUIS, MO 63167 USA

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 1996年 / 303卷 / 03期

关键词：

carrageenan; inflammation; peroxynitrite;

D O I：

10.1016/0014-2999(96)00140-9

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The role of peroxynitrite generated from nitric oxide and superoxide anion was investigated in a model of acute inflammation induced by the injection of carrageenan into the rat hind paw. Paw edema was inhibited 8 h following the administration of carrageenan by N-iminoethyl-L-lysine (3-30 mg/kg, n = 6) or aminoguanidine (30-300 mg/kg, n = 6), two selective inhibitors of inducible nitric oxide synthase and by recombinant human Cu/Zn superoxide dismutase coupled to polyethyleneglycol (12 X 10(3) U/kg, n = 6, P < 0.001). Moreover, at the same time point following carrageenan administration, intense immunoreactive staining for nitrotyrosine (a marker of peroxynitrite formation) was detected. Our results suggest that the generation of nitric oxide, superoxide anion and peroxynitrite contributes to the edema observed in this acute model of inflammation.

引用

收藏

页码：217 / 220

页数：4

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