Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium-rich environments and recovery of genes using integron-specific primers

被引：15

作者：

Ghauri, MA

Khalid, AM

Grant, S

Heaphy, S

Grant, WD

机构：

[1] Univ Leicester, Dept Microbiol & Immunol, Leicester LE1 9HN, Leics, England

[2] Natl Inst Biotechnol & Genet Engn, Faisalabad, Pakistan

来源：

EXTREMOPHILES | 2003年 / 7卷 / 05期

关键词：

acidophilic moderate thermophiles; gene cassettes; integrons; 16S rDNA analysis; uranium rich environments;

D O I：

10.1007/s00792-003-0354-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The isolation and phylogenetic characterization of acidophilic moderate thermophilic bacteria from different locations of uranium mines and a uranium processing mill in Pakistan is reported. The dominant culturable bacteria found were related to Sulfobacillus thermosulfidooxidans in all the samples analyzed. Different strains displayed different levels of identity (95-97%) tol6S rDNA of known strains of this species, indicating group heterogeneity. Genomic DNA from five isolates was subjected to amplification using integron-specific primers HS286 and HS287. Recovery of different integron-linked genes from one of the isolates indicated the usefulness of this approach for gene mining in place of traditional gene recovery methodologies.

引用

页码：341 / 345

页数：5

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