Proteome analysis for the identification of tumor-associated biomarkers in gastrointestinal cancer

被引：17

作者：

Fels, LM

Buschmann, T

Meuer, J

Reymond, MA

Lamer, S

Röcken, C

Ebert, MPA

机构：

[1] Europroteome AG, D-16761 Hennigsdorf, Germany

[2] Otto Von Guericke Univ, Dept Surg, Magdeburg, Germany

[3] Otto Von Guericke Univ, Inst Pathol, Magdeburg, Germany

[4] Otto Von Guericke Univ, Dept Gastroenterol Hepatol & Infect Dis, Magdeburg, Germany

来源：

DIGESTIVE DISEASES | 2003年 / 21卷 / 04期

关键词：

proteomics; gastric neoplasms; colon neoplasms; two-dimensional gel electrophoresis; SELDI;

D O I：

10.1159/000075351

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Gastrointestinal cancers are usually diagnosed at advanced stages, making a curative treatment difficult. Biomarkers can help to overcome this problem by allowing earlier diagnosis, and thus better therapy. Proteomics tools are novel technologies to identify such biomarkers. This review summarizes advances in biomarker detection using two-dimensional gel electrophoresis (2D-PAGE), chromatography and mass spectrometry technologies. 2D-PAGE combined with mass spectrometry has led to the identification of several differentially expressed proteins in cancer tissue. However, for serum analysis, 2D-PAGE has severe limitations. For serum-based cancer diagnosis, surface-enhanced laser desorption-ionization time-of-flight (SELDI-TOF(TM)) mass spectrometry is a promising new technology. The potential of proteins identified with this technology as novel cancer biomarkers still needs to be confirmed in clinical trials. Copyright (C) 2003 S. Karger AG, Basel.

引用

页码：292 / 298

页数：7

共 38 条

[1]

Adam BL, 2002, CANCER RES, V62, P3609

[2] New technologies for the identification of markers for early detection of ovarian cancer [J].